Figure 1

Experimental procedure for C. cellulovorans exoproteome analysis. Proteome analysis was performed as previously described (Matsui et al. 2013). Proteins in the culture supernatant of C. cellulovorans grown in the presence of cellobiose (Cb), bagasse, corn germ, or rice straw were individually reductive-alkylated and digested with trypsin, and tryptic fragments were labeled with tandem mass tags (TMTs). The labeled peptides were mixed and injected into the LC-MS/MS system with a long monolithic column for mass measurement, and the data collected were used for protein quantification.