Unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway

AMB Express

Table 3 The kinetic parameters of 3-dehydroquinate dehydratases

Codes for DHQases*	V _max (μmol/L/s)	K _m (μmol/L)	k _cat ( s ⁻¹ )	k _cat /K _m (L/μmol/s)	Specific Activity (U/mg)**
3001	2.56	281.43	52.26	0.19	119.14
3002	4.57	187.68	29.53	0.16	57.34
3003	1.34	210.71	13.53	0.06	34.72
3004	——	——	——	——	——
3005	15.50	870.37	208.96	0.24	93.91
3006	6.39	478.67	102.15	0.21	187.69
3007	2.43	777.75	77.06	0.10	84.95
3008	2.46	624.52	23.96	0.04	46.37
3009	4.24	203.24	31.67	0.16	75.06
3010	0.80	202.55	10.59	0.05	9.39
3011	0.57	158.79	7.15	0.05	15.06
3012	66.67	448.50	185.43	0.41	369.99
3013	7.04	159.89	4.63	0.03	86.50
3014	3.60	151.51	3.83	0.03	91.93
3015	4.18	46.14	10.00	0.22	67.71
3016	12.42	141.53	211.83	1.50	501.87
3017	8.25	1218.56	11.92	0.01	30.19
3018	0.68	400.82	9.06	0.02	15.64
3019	——	——	——	——	——
3020	0.96	107.07	10.46	0.10	62.27
3021	2.16	133.97	5.33	0.04	15.90
3022	8.26	601.64	119.98	0.20	195.81
3023	2.86	67.88	42.20	0.62	68.99
3024	6.23	526.07	75.11	0.14	131.08
3025	5.89	368.46	59.61	0.16	117.89
3026	2.95	304.54	3.80	0.01	5.16
3027	6.00	255.54	50.20	0.20	116.07
3028	1.16	180.72	6.88	0.04	17.66
3029	——	——	——	——	——
3030	1.14	92.76	3.86	0.04	8.58
3031	1.45	37.16	5.95	0.16	19.82
3032	12.35	617.28	78.87	0.13	105.18
3033	2.64	150.25	57.84	0.38	155.93
3034	3.78	345.90	37.97	0.11	71.87
3035	11.14	2226.50	192.27	0.09	136.57
3036	9.64	131.63	102.70	0.78	192.10
3037	3.87	119.97	73.18	0.61	365.73
3038	——	——	——	——	——

*The first digit of the code represents DHQase catalyzes the 3rd step of shikimate pathway, and the following digits are the order when that DHQase was selected. **Specific activities were determined at substrate concentration of 0.5 mM. “-” indicated no activity was detected.