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Table 3 The kinetic parameters of 3-dehydroquinate dehydratases

From: Unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway

Codes for DHQases* V max (μmol/L/s) K m (μmol/L) k cat ( s −1 ) k cat /K m (L/μmol/s) Specific Activity** (U/mg)
3001 2.56 281.43 52.26 0.19 119.14
3002 4.57 187.68 29.53 0.16 57.34
3003 1.34 210.71 13.53 0.06 34.72
3004 —— —— —— —— ——
3005 15.50 870.37 208.96 0.24 93.91
3006 6.39 478.67 102.15 0.21 187.69
3007 2.43 777.75 77.06 0.10 84.95
3008 2.46 624.52 23.96 0.04 46.37
3009 4.24 203.24 31.67 0.16 75.06
3010 0.80 202.55 10.59 0.05 9.39
3011 0.57 158.79 7.15 0.05 15.06
3012 66.67 448.50 185.43 0.41 369.99
3013 7.04 159.89 4.63 0.03 86.50
3014 3.60 151.51 3.83 0.03 91.93
3015 4.18 46.14 10.00 0.22 67.71
3016 12.42 141.53 211.83 1.50 501.87
3017 8.25 1218.56 11.92 0.01 30.19
3018 0.68 400.82 9.06 0.02 15.64
3019 —— —— —— —— ——
3020 0.96 107.07 10.46 0.10 62.27
3021 2.16 133.97 5.33 0.04 15.90
3022 8.26 601.64 119.98 0.20 195.81
3023 2.86 67.88 42.20 0.62 68.99
3024 6.23 526.07 75.11 0.14 131.08
3025 5.89 368.46 59.61 0.16 117.89
3026 2.95 304.54 3.80 0.01 5.16
3027 6.00 255.54 50.20 0.20 116.07
3028 1.16 180.72 6.88 0.04 17.66
3029 —— —— —— —— ——
3030 1.14 92.76 3.86 0.04 8.58
3031 1.45 37.16 5.95 0.16 19.82
3032 12.35 617.28 78.87 0.13 105.18
3033 2.64 150.25 57.84 0.38 155.93
3034 3.78 345.90 37.97 0.11 71.87
3035 11.14 2226.50 192.27 0.09 136.57
3036 9.64 131.63 102.70 0.78 192.10
3037 3.87 119.97 73.18 0.61 365.73
3038 —— —— —— —— ——
  1. *The first digit of the code represents DHQase catalyzes the 3rd step of shikimate pathway, and the following digits are the order when that DHQase was selected. **Specific activities were determined at substrate concentration of 0.5 mM. “-” indicated no activity was detected.