Table 1 S. cerevisiae strains and plasmids used in this work
Strains | Genotype description | Reference |
|---|---|---|
CEN.PK 113-11C | MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 | (van Dijken et al. [2000]) |
CEN.PK 113-11C; p426; p423 (CN1) | MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 p426TEF1 p423TEF1 | This work |
fps1∆; p426-GapN; p423-UTR1 (CN2) | MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p426TEF1-GapN p423TEF1-UTR1 | This work |
fps1∆; p426-GapN; p413-UTR1 (CN3) | MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p426TEF1-GapN p413TEF1-UTR1 | This work |
fps1∆; p416-GapN; p423-UTR1 (CN4) | MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p416TEF1-GapN p423TEF1-UTR1 | This work |
fps1∆; p416-GapN; p413-UTR1 (CN5) | MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p416TEF1-GapN p413TEF1-UTR1 | This work |
fps1∆; p426; p423 (CN6) | MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p426TEF1 p423TEF1 | This work |
Plasmids | ||
pCIChE-KK004-Gap | neor, integrative plasmid | (Kocharin [2013]) |
p426TEF1 | URA3, 2 μ plasmid, TEF1 promoter, CYC1 terminator | (Mumberg et al. [1995]) |
p416TEF1 | URA3, centromeric plasmid, TEF1 promoter, CYC1 terminator | (Mumberg et al. [1995]) |
p423TEF1 | HIS3, 2 μ plasmid, TEF1 promoter, CYC1 terminator | (Mumberg et al. [1995]) |
p413TEF1 | HIS3, centromeric plasmid, TEF1 promoter, CYC1 terminator | (Mumberg et al. [1995]) |
p426TEF1-GapN | URA3, 2 μ plasmid, gapN from S. mutans | This work |
p416TEF1-GapN | URA3, centromeric plasmid, gapN from S. mutans | This work |
p423TEF1-UTR1 | HIS3, 2 μ plasmid, UTR1 | This work |
p413TEF1-UTR1 | HIS3, centromeric plasmid, UTR1 | This work |