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Table 1 S. cerevisiae strains and plasmids used in this work

From: Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing

Strains Genotype description Reference
CEN.PK 113-11C MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 (van Dijken et al. [2000])
CEN.PK 113-11C; p426; p423 (CN1) MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 p426TEF1 p423TEF1 This work
fps1∆; p426-GapN; p423-UTR1 (CN2) MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p426TEF1-GapN p423TEF1-UTR1 This work
fps1∆; p426-GapN; p413-UTR1 (CN3) MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p426TEF1-GapN p413TEF1-UTR1 This work
fps1∆; p416-GapN; p423-UTR1 (CN4) MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p416TEF1-GapN p423TEF1-UTR1 This work
fps1∆; p416-GapN; p413-UTR1 (CN5) MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p416TEF1-GapN p413TEF1-UTR1 This work
fps1∆; p426; p423 (CN6) MAT a his3Δ 1 ura3-52 MAL2-8cSUC2 fps1∆ p426TEF1 p423TEF1 This work
Plasmids   
pCIChE-KK004-Gap neor, integrative plasmid (Kocharin [2013])
p426TEF1 URA3, 2 μ plasmid, TEF1 promoter, CYC1 terminator (Mumberg et al. [1995])
p416TEF1 URA3, centromeric plasmid, TEF1 promoter, CYC1 terminator (Mumberg et al. [1995])
p423TEF1 HIS3, 2 μ plasmid, TEF1 promoter, CYC1 terminator (Mumberg et al. [1995])
p413TEF1 HIS3, centromeric plasmid, TEF1 promoter, CYC1 terminator (Mumberg et al. [1995])
p426TEF1-GapN URA3, 2 μ plasmid, gapN from S. mutans This work
p416TEF1-GapN URA3, centromeric plasmid, gapN from S. mutans This work
p423TEF1-UTR1 HIS3, 2 μ plasmid, UTR1 This work
p413TEF1-UTR1 HIS3, centromeric plasmid, UTR1 This work
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