E. coli | ||
---|---|---|
DH5α | F−thi-1 endA 1 hsdr 17(r−, m−) supE 44 ∆lacU 169 (Φ80lacZ∆M15) recA 1 gyrA 96 relA 1 | (Hanahan [1983]) |
C. glutamicum | ||
MB001 | ATCC 13032 with in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752), and CGP3 (cg1890-cg2071) | (Baumgart et al. [2013]) |
CIT0 | MB001 with ∆argF, ∆argG, ∆argR | This study |
CIT1 | CIT0 carrying the pVWEx-argFBfbr vector | This study |
Plasmids | ||
pEKEx3 | SpecR, Ptac, lacIq | (Stansen et al. [2005]) |
pVWEx1 | KanR, Ptac, lacIq | (Peters-Wendisch et al. [2001]) |
pEC-XT99A | TetR, Ptrc, lacIq | (Kirchner and Tauch [2003]) |
pK19∆argFR | KanR, pk19mobsacB with the deletion construct of genes argFR | (Schneider et al. [2011]) |
pK19∆argG | KanR, pk19mobsacB with the deletion construct of genes argG | This study |
pEKEx3- argBfbr | SpecR, pEKEx3 carrying argB from C. glutamicum ATCC 13032 with amino acid exchanges A49VM54V | (Schneider et al. [2011]) |
pVWEx1-argF | KanR, pVWEx1 carrying argF from C. glutamicum ATCC 13032 | This study |
pVWEx1-argFG | KanR, pVWEx1 carrying argF and argG from C. glutamicum ATCC 13032 | This study |
pVWEx1-argFBfbr | KanR, pVWEx1 carrying argF from C. glutamicum ATCC 13032 and argBfbr from pEKEx3- argBfbr | This study |
pEKEx3-nagB | SpecR, pEKEx3 carrying nagB from C. glutamicum ATCC 13032 | (Uhde et al. [2013]) |
pEKEx3-xylAB | SpecR, pEKEx3 carrying xylA from Xanthomonas campestris XCC1758 and xylB from C. glutamicum ATCC 13032 | (Meiswinkel et al. [2013a]) |
pAMY | TetR, pEC-XT99A carrying amy from Streptomyces griseus IMRU 3570 | (Seibold et al. [2006]) |