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Table 1 Strains and plasmids used in this study

From: L-citrulline production by metabolically engineered Corynebacterium glutamicum from glucose and alternative carbon sources

E. coli

DH5α

Fthi-1 endA 1 hsdr 17(r, m) supE 44 ∆lacU 169 (Φ80lacZ∆M15) recA 1 gyrA 96 relA 1

(Hanahan [1983])

C. glutamicum

MB001

ATCC 13032 with in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752), and CGP3 (cg1890-cg2071)

(Baumgart et al. [2013])

CIT0

MB001 with ∆argF, ∆argG, ∆argR

This study

CIT1

CIT0 carrying the pVWEx-argFBfbr vector

This study

Plasmids

pEKEx3

SpecR, Ptac, lacIq

(Stansen et al. [2005])

pVWEx1

KanR, Ptac, lacIq

(Peters-Wendisch et al. [2001])

pEC-XT99A

TetR, Ptrc, lacIq

(Kirchner and Tauch [2003])

pK19∆argFR

KanR, pk19mobsacB with the deletion construct of genes argFR

(Schneider et al. [2011])

pK19∆argG

KanR, pk19mobsacB with the deletion construct of genes argG

This study

pEKEx3- argBfbr

SpecR, pEKEx3 carrying argB from C. glutamicum ATCC 13032 with amino acid exchanges A49VM54V

(Schneider et al. [2011])

pVWEx1-argF

KanR, pVWEx1 carrying argF from C. glutamicum ATCC 13032

This study

pVWEx1-argFG

KanR, pVWEx1 carrying argF and argG from C. glutamicum ATCC 13032

This study

pVWEx1-argFBfbr

KanR, pVWEx1 carrying argF from C. glutamicum ATCC 13032 and argBfbr from pEKEx3- argBfbr

This study

pEKEx3-nagB

SpecR, pEKEx3 carrying nagB from C. glutamicum ATCC 13032

(Uhde et al. [2013])

pEKEx3-xylAB

SpecR, pEKEx3 carrying xylA from Xanthomonas campestris XCC1758 and xylB from C. glutamicum ATCC 13032

(Meiswinkel et al. [2013a])

pAMY

TetR, pEC-XT99A carrying amy from Streptomyces griseus IMRU 3570

(Seibold et al. [2006])