Skip to main content

Table 1 Strains and plasmids used in this study

From: L-citrulline production by metabolically engineered Corynebacterium glutamicum from glucose and alternative carbon sources

E. coli
DH5α Fthi-1 endA 1 hsdr 17(r, m) supE 44 ∆lacU 169 (Φ80lacZ∆M15) recA 1 gyrA 96 relA 1 (Hanahan [1983])
C. glutamicum
MB001 ATCC 13032 with in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752), and CGP3 (cg1890-cg2071) (Baumgart et al. [2013])
CIT0 MB001 with ∆argF, ∆argG, ∆argR This study
CIT1 CIT0 carrying the pVWEx-argFBfbr vector This study
Plasmids
pEKEx3 SpecR, Ptac, lacIq (Stansen et al. [2005])
pVWEx1 KanR, Ptac, lacIq (Peters-Wendisch et al. [2001])
pEC-XT99A TetR, Ptrc, lacIq (Kirchner and Tauch [2003])
pK19∆argFR KanR, pk19mobsacB with the deletion construct of genes argFR (Schneider et al. [2011])
pK19∆argG KanR, pk19mobsacB with the deletion construct of genes argG This study
pEKEx3- argBfbr SpecR, pEKEx3 carrying argB from C. glutamicum ATCC 13032 with amino acid exchanges A49VM54V (Schneider et al. [2011])
pVWEx1-argF KanR, pVWEx1 carrying argF from C. glutamicum ATCC 13032 This study
pVWEx1-argFG KanR, pVWEx1 carrying argF and argG from C. glutamicum ATCC 13032 This study
pVWEx1-argFBfbr KanR, pVWEx1 carrying argF from C. glutamicum ATCC 13032 and argBfbr from pEKEx3- argBfbr This study
pEKEx3-nagB SpecR, pEKEx3 carrying nagB from C. glutamicum ATCC 13032 (Uhde et al. [2013])
pEKEx3-xylAB SpecR, pEKEx3 carrying xylA from Xanthomonas campestris XCC1758 and xylB from C. glutamicum ATCC 13032 (Meiswinkel et al. [2013a])
pAMY TetR, pEC-XT99A carrying amy from Streptomyces griseus IMRU 3570 (Seibold et al. [2006])