Figure 3
From: Inhibitory effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function
Western blot analysis for the confirmation of ERK1/2 phosphorylation. MH7A cells were transfected with plasmids encoding affibodies. After 24 h of culture, the cells were stimulated with TNF-α (100 ng/mL) and cultured for an additional 24-48 h. Each soluble fraction of MH7A cells harboring plasmid-encoded affibody molecules was analyzed via western blot for the expression of p-ERK and actin at 16 and 24 h after TNF-α stimulation. Representative data are shown for p-ERK and actin expression by MH7A cells.