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TRP1_gpd1Over_f
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Forward primer for amplification of TRP1 (p424 as template) with GPD1 overhangs
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TCCACAAACACAAATATTGATAATATAAAGAACGACATTACTATATATATAATATAG
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TRP1_gpd1Over_r
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Reverse primer for amplification of TRP1 (p424 as template) with GPD1 overhangs
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AGTATGATATGTTATCTTTCTCCAATAAATAGGCAAGTGCACAAACAATAC
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TRP1_gpd1_veri_f
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Amplification of the 5' region of GPD1 (CEN.PK113-7D as template)
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TTCCATTCACATATCGTCTTTGG
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+ Verification of GPD1 deletions
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5-OEGpd1_r
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Amplification of the 5' region of GPD1 (CEN.PK113-7D as template)
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TATATTATCAATATTTGTGTTTGTGG
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3-OEGpd1_f
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Amplification of the 3' region of GPD1 (CEN.PK113-7D as template)
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TTTATTGGAGAAAGATAACATATCATAC
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3-OEGpd1_r
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Amplification of the 3' region of GPD1 (CEN.PK113-7D as template)
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ATTTTCTTAGGACGCCGCAAAATATC
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TRP1_gpd1_veri_r
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Verification of GPD1 deletions
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GAGGAACTCTTGGTATTCTTGCC
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LEU2_gpd2Over_f
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Forward primer for amplification of LEU2 (p425 as template) with GPD2 overhangs
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TTCCTTTTCCTTCGCTCCCCTTCCTTATCATCGACTACGTCGTAAGGCCG
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LEU2_gpd2Over_r
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Forward primer for amplification of LEU2 (p425 as template) with GPD2 overhangs
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GATCAGAGGGGGAGGGGGGGGGAGAGTGTCGAGGAGAACTTCTAGTATATC
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LEU2_gpd2OO_f
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Forward primer for nested PCR for adding homology regions to the GPD2 deletion casette
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GTATTTTGGTAGATTCAATTCTCTTTCCCTTTCCTTTTCCTTCGCTCCC
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LEU2_gpd2OO_r
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Reverse primer for nested PCR for adding homology regions to the GPD2 deletion casette
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AAATTGGTTGGGGGAAAAAGAGGCAACAGGAAAGATCAGAGGGGGAGGG
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LEU2_gpd2_veri_f
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Verification of GPD2 deletions
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CGTGTATCTTCTAAGATTCAGTC
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LEU2_gpd2_veri_r
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Verification of GPD2 deletions
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CTAATGGCTCAACGTGATAAGG
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GPD2p_XbaI_r
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Cloning of GPD2 from CEN.PK113-7D
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CTTCTAGATTGATAAGGAAGGGGAG
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GPD2p_SacI_f
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Cloning of GPD2 from CEN.PK113-7D
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TCGAGCTCCGCAATGTTTCGTTGG
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yEGFP_XbaI_f
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Cloning of yEGFP3 from pyEGFP3
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AATCTAGAAGCATTAAAAAATGTCTAAAGGTGAAG
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yEGFP_PstI_r
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Cloning of yEGFP3 from pyEGFP3
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TTCCTGCAGAGTTATTTGTACAATTCATCCATAC
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HIS3_flank_f
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Cloning of HIS 3 - Forward primer
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CCAGGTATCGTTTGAACACGG
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HIS3_flank_r
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Cloning of HIS 3 - Reverse primer
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GCTCAGTTCAGCCATAATATG
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LEU2_flank_f
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Cloning of LEU2 - Forward primer
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GGATAATTATACTCTATTTCTCAAC
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LEU2_r
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Cloning of LEU2 - Reverse primer
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TTAAGCAAGGATTTTCTTAAC
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TRP1_f
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Cloning of TRP1 - Forward primer
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ATGTCTGTTATTAATTTCAC
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TRP1_r
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Cloning of TRP1- Reverse primer
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CTATTTCTTAGCATTTTTG
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YIplac211_f
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Cloning of GPD2 p-yEGFP3-PGK 1t
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TTTATCTTCGTTTCCTGC
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YIplac211_r
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Cloning of GPD2 p-yEGFP3-PGK1 t
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AAAACTGTATTATAAGTAAATG
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HIS3_O_f
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Cloning of HIS3- expression cassette, for change of auxotrophic marker.
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TTATAATACAGTTTTCCAGGTATCGTTTGAACACGG
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HIS3_O_r
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Cloning of HIS3-expression cassette, for change of auxotrophic marker.
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GGAAACGAAGATAAATCGCTCAGTTCAGCCATAATATG
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