Name | Description | Sequence |
---|---|---|
TRP1_gpd1Over_f | Forward primer for amplification of TRP1 (p424 as template) with GPD1 overhangs | TCCACAAACACAAATATTGATAATATAAAGAACGACATTACTATATATATAATATAG |
TRP1_gpd1Over_r | Reverse primer for amplification of TRP1 (p424 as template) with GPD1 overhangs | AGTATGATATGTTATCTTTCTCCAATAAATAGGCAAGTGCACAAACAATAC |
TRP1_gpd1_veri_f | Amplification of the 5' region of GPD1 (CEN.PK113-7D as template) | TTCCATTCACATATCGTCTTTGG |
+ Verification of GPD1 deletions | ||
5-OEGpd1_r | Amplification of the 5' region of GPD1 (CEN.PK113-7D as template) | TATATTATCAATATTTGTGTTTGTGG |
3-OEGpd1_f | Amplification of the 3' region of GPD1 (CEN.PK113-7D as template) | TTTATTGGAGAAAGATAACATATCATAC |
3-OEGpd1_r | Amplification of the 3' region of GPD1 (CEN.PK113-7D as template) | ATTTTCTTAGGACGCCGCAAAATATC |
TRP1_gpd1_veri_r | Verification of GPD1 deletions | GAGGAACTCTTGGTATTCTTGCC |
LEU2_gpd2Over_f | Forward primer for amplification of LEU2 (p425 as template) with GPD2 overhangs | TTCCTTTTCCTTCGCTCCCCTTCCTTATCATCGACTACGTCGTAAGGCCG |
LEU2_gpd2Over_r | Forward primer for amplification of LEU2 (p425 as template) with GPD2 overhangs | GATCAGAGGGGGAGGGGGGGGGAGAGTGTCGAGGAGAACTTCTAGTATATC |
LEU2_gpd2OO_f | Forward primer for nested PCR for adding homology regions to the GPD2 deletion casette | GTATTTTGGTAGATTCAATTCTCTTTCCCTTTCCTTTTCCTTCGCTCCC |
LEU2_gpd2OO_r | Reverse primer for nested PCR for adding homology regions to the GPD2 deletion casette | AAATTGGTTGGGGGAAAAAGAGGCAACAGGAAAGATCAGAGGGGGAGGG |
LEU2_gpd2_veri_f | Verification of GPD2 deletions | CGTGTATCTTCTAAGATTCAGTC |
LEU2_gpd2_veri_r | Verification of GPD2 deletions | CTAATGGCTCAACGTGATAAGG |
GPD2p_XbaI_r | Cloning of GPD2 from CEN.PK113-7D | CTTCTAGATTGATAAGGAAGGGGAG |
GPD2p_SacI_f | Cloning of GPD2 from CEN.PK113-7D | TCGAGCTCCGCAATGTTTCGTTGG |
yEGFP_XbaI_f | Cloning of yEGFP3 from pyEGFP3 | AATCTAGAAGCATTAAAAAATGTCTAAAGGTGAAG |
yEGFP_PstI_r | Cloning of yEGFP3 from pyEGFP3 | TTCCTGCAGAGTTATTTGTACAATTCATCCATAC |
HIS3_flank_f | Cloning of HIS 3 - Forward primer | CCAGGTATCGTTTGAACACGG |
HIS3_flank_r | Cloning of HIS 3 - Reverse primer | GCTCAGTTCAGCCATAATATG |
LEU2_flank_f | Cloning of LEU2 - Forward primer | GGATAATTATACTCTATTTCTCAAC |
LEU2_r | Cloning of LEU2 - Reverse primer | TTAAGCAAGGATTTTCTTAAC |
TRP1_f | Cloning of TRP1 - Forward primer | ATGTCTGTTATTAATTTCAC |
TRP1_r | Cloning of TRP1- Reverse primer | CTATTTCTTAGCATTTTTG |
YIplac211_f | Cloning of GPD2 p-yEGFP3-PGK 1t | TTTATCTTCGTTTCCTGC |
YIplac211_r | Cloning of GPD2 p-yEGFP3-PGK1 t | AAAACTGTATTATAAGTAAATG |
HIS3_O_f | Cloning of HIS3- expression cassette, for change of auxotrophic marker. | TTATAATACAGTTTTCCAGGTATCGTTTGAACACGG |
HIS3_O_r | Cloning of HIS3-expression cassette, for change of auxotrophic marker. | GGAAACGAAGATAAATCGCTCAGTTCAGCCATAATATG |