Table 1 Plasmids and yeast strains used in the present study
Strains and plasmids | Relevant features | Reference |
|---|---|---|
Plasmids | ||
YIplac211 | URA3 | (Gietz and Sugino [1988]) |
p424 | TRP1 Mumberg vector | (Mumberg et al. [1995]) |
P425 | LEU2 Mumberg vector | (Mumberg et al. [1995]) |
YEplacHXT | URA3 | (Karhumaa et al. [2005]) |
pYGFP3 | URA3 ADH1p-yEGFP3-ADH1t | (Cormack et al. [1997]) |
YIpJK01 | HIS3 GPD2p-yEGFP3-PGK1t | This study |
YEpJK01 | URA3 GPD2p-yEGFP3-PGK1t | This study |
YIpOB8 | URA3 TDH3p-XYL1-ADH1t PGK1p-XYL2-PGK1t | (Bengtsson et al. [2009]) |
YIpDR7 | URA3 TDH3p-XYL1(N272D)-ADH1t PGK1p-XYL2-PGK1t | (Runquist et al. [2010]) |
YIpCR01 | URA3 TDH3p-XYL1-ADH1t | This study |
YIpCR03 | URA3 TDH3p-XYL1 (N272D)-ADH1t | This study |
S. cerevisiae strains | ||
CEN.PK2-1C | MATa ura3-52 trp1-289 leu2-3,112 his3Δ 1 MAL2-8C SUC2 | EUROSCARF |
CEN.PK113-7D | MATα, MAL2-8 c SUC2 | EUROSCARF |
TMB4120 | CEN.PK2-1C YEpJK01 trp1::TRP1 leu2::LEU2 his3::HIS3 | This study |
TMB4121 | CEN.PK2-1C YEpJK01 gpd1::TRP1 leu2::LEU2 his3::HIS3 | This study |
TMB4122 | CEN.PK2-1C YEpJK01 trp1::TRP1 gpd2::LEU2 his3::HIS3 | This study |
TMB4123 | CEN.PK2-1C YEpJK01 gpd1::TRP1 gpd2::LEU2 his3::HIS3 | This study |
TMB4132 | CEN.PK2-1C his3::YipJK01 trp1::TRP1 leu2::LEU2 ura3- | This study |
TMB4133 | CEN.PK2-1C his3::YipJK01 gpd1::TRP1 gpd2:LEU2 ura3- | This study |
TMB4140 | TMB4132 ura3::YIplac211 | This study |
TMB4141 | TMB4132 ura3::YIpCR01 | This study |
TMB4143 | TMB4132 ura3::YIpCR03 | This study |
TMB4144 | TMB4133 ura3::YIplac211 | This study |
TMB4145 | TMB4133 ura3::YIpCR01 | This study |
TMB4147 | TMB4133 ura3::YIpCR03 | This study |