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Table 1 Efficiency of protoplast isolation from A95 cells using different cell-wall lysing enzymes

From: Genetic transformation of Knufia petricola A95 - a model organism for biofilm-material interactions

Enzymes used for protoplast isolation

Starting number of A95 cells (cfu.mL-1)

Number of protoplasts isolated.mL-1

Lysing enzymes from Trichoderma harzianum

2 × 107

2.0 × 106

β-Glucanase, Vinoflow, Yatalase

2 × 107

1.9 × 106

Driselase, β-Glucanase, Lyticase

2 × 107

1.1 × 106

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