A95 transformants. A- three weeks after PEG mediated transformation of A95 protoplasts with pCB1004, single A95 colonies were observed on malt-extract agar plates containing hygromycin. B- PCR products of the hph-gene (1,019 bp) seen on agarose gels. Polymerase chain reaction detection of the hph-gene from DNA of five independent putative transformants confirmed integration of hph into the genome of A95 transformants. Arrow indicates location of hph DNA bands.