Construction of a biodynamic model for Cry protein production studies

Navarro-Mtz, Ana Karin; Pérez-Guevara, Fermín

doi:10.1186/s13568-014-0079-y

AMB Express

Table 3 Parameters culture of Bacillus thuringiensis reported in the literature

From: Construction of a biodynamic model for Cry protein production studies

Biomass	PHB	Cry	μ_max^a	Y_X/S^b	Y_Cry/X^c	Comment	Reference
8.41 to 32.19 g/L		0.16-1.24 g/L	0.19 - 1.09	0.43 - 0.67	0.02 to 0.03	Same culture conditions and techniques to this work.	Previous experiments^g
6.78 to 25.70 g/L^f	0.52-1.1 mg/L	0.1-1.2 g/L	0.37 - 0.63^f	0.39 - 0.68^f	0.01 to 0.04	Same culture conditions and techniques to this work.	Navarro et al. ([2006])
2.57 to 13.64 g/L	8.99 to 13.17%^e	0.17-0.77 g/L	0.78 - 1.06	0.16 - 0.25	0.06 to 0.05	Culture medium with different initial nutrient concentration. Biomass detection by direct counts technique. PHB detection by CG Technique. Cry detection by SDS-PAGE technique.	Present work
1.65x10⁹ CFU/cm³ 3.79 g/L^d		2.67 g/L			0.7	Starch processing wastewater medium. Biomass detection by CFU technique. Cry detection by SDS-PAGE technique.	Chang et al. ([2008])
16 g/L		12.8 - 15.7 g/L	0.69-1.2		0.8 to 0.98	Fed Batch with balanced flux of substrate. Biomass detection by UV-Vis technique. Cry detection by ELISA technique.	Anderson and Jayaraman ([2005])
3.8x10⁸ CFU/cm³ⁱ 0.87 g/L^d		1.043 g/Lⁱ			1.19	Culture with SodAcet as pH control agent. Biomass detection by CFU technique. Cry detection by alkaline solubilization and Bradford method.	Dang Vu et al. ([2009])
0.34 to 1.02 g/L	7.54 to 29.41%^e					Nutrient broth as culture medium. PHB detection by UV-Vis technique.	Aslim et al. ([2002])
2.78-3.3 g/L		0.28 - 0.415 g/L				Different oxygen supply. Biomass detection by dry weight technique. Cry detection by alkaline solubilization and Lowry method.	Avignone-Rossa et al. (1992)
5.6-20 g/L^h			0.58-0.8	0.3-0.7		Culture medium with different initial nutrient concentration. Culture under similar conditions to this work.	Amicarelli et al. (2010)
1.64-4.78 g/L						Culture media with different peptone + yeast extract concentration. Biomass detection by dry weight technique.	Prabakaran and Hoti (2008)
			0.81	2.25^g		Culture under conditions of carbon limitation.	Popovic et al. ([2001])
			0.58-0.8	0.37-0.7		Culture in intermittent fed-batch with total cell retention. Culture medium with different glucose and yeast extract concentration.	Atehortúa et al. (2007)
8.5 - 15.9 g/L			0.79-1.1	0.41-0.8		Similar conditions of culture to this work. Biomass detection by UV-Vis.	Berbert-Molina et al. ([2008])
		0.36-2.66 g/L				Similar conditions of culture to this work.	Farrera et al. ([1998])
			0.95			Similar conditions of culture to this work.	Holmberg and Sievänen ([1980])
			0.53			Similar conditions of culture to this work.	Rivera et al. ([1999])

^aMaximum specific growth rate (1/h).
^bYield of biomass from glucose (g biomass/g glucose).
^cYield of Cry from biomass (g Cry/g biomass). This value was calculated with the biomass and Cry protein concentration reported.
^dThis value was calculated considering that the weight of one B. thuringiensis cell is 2.3 pg (Rodriguez and de la Torre, 1996).
^ePHB percentage of dry cell weight.
^fUnpublished data.
^gYield of active biomass from limiting substrate.
^hThis value was calculated with the glucose concentration in the media and the yield of biomass from glucose reported by the author.

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