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Table 3 Parameters culture of Bacillus thuringiensis reported in the literature

From: Construction of a biodynamic model for Cry protein production studies

Biomass PHB Cry μmaxa YX/Sb YCry/Xc Comment Reference
8.41 to 32.19 g/L   0.16-1.24 g/L 0.19 - 1.09 0.43 - 0.67 0.02 to 0.03 Same culture conditions and techniques to this work. Previous experimentsg
6.78 to 25.70 g/Lf 0.52-1.1 mg/L 0.1-1.2 g/L 0.37 - 0.63f 0.39 - 0.68f 0.01 to 0.04 Same culture conditions and techniques to this work. Navarro et al. ([2006])
2.57 to 13.64 g/L 8.99 to 13.17%e 0.17-0.77 g/L 0.78 - 1.06 0.16 - 0.25 0.06 to 0.05 Culture medium with different initial nutrient concentration. Biomass detection by direct counts technique. PHB detection by CG Technique. Cry detection by SDS-PAGE technique. Present work
1.65x109 CFU/cm3
3.79 g/Ld
  2.67 g/L    0.7 Starch processing wastewater medium. Biomass detection by CFU technique. Cry detection by SDS-PAGE technique. Chang et al. ([2008])
16 g/L   12.8 - 15.7 g/L 0.69-1.2   0.8 to 0.98 Fed Batch with balanced flux of substrate. Biomass detection by UV-Vis technique. Cry detection by ELISA technique. Anderson and Jayaraman ([2005])
3.8x108 CFU/cm3i
0.87 g/Ld
  1.043 g/Li    1.19 Culture with SodAcet as pH control agent. Biomass detection by CFU technique. Cry detection by alkaline solubilization and Bradford method. Dang Vu et al. ([2009])
0.34 to 1.02 g/L 7.54 to 29.41%e      Nutrient broth as culture medium. PHB detection by UV-Vis technique. Aslim et al. ([2002])
2.78-3.3 g/L   0.28 - 0.415 g/L     Different oxygen supply. Biomass detection by dry weight technique. Cry detection by alkaline solubilization and Lowry method. Avignone-Rossa et al. (1992)
5.6-20 g/Lh    0.58-0.8 0.3-0.7   Culture medium with different initial nutrient concentration. Culture under similar conditions to this work. Amicarelli et al. (2010)
1.64-4.78 g/L       Culture media with different peptone + yeast extract concentration. Biomass detection by dry weight technique. Prabakaran and Hoti (2008)
    0.81 2.25g   Culture under conditions of carbon limitation. Popovic et al. ([2001])
    0.58-0.8 0.37-0.7   Culture in intermittent fed-batch with total cell retention. Culture medium with different glucose and yeast extract concentration. Atehortúa et al. (2007)
8.5 - 15.9 g/L    0.79-1.1 0.41-0.8   Similar conditions of culture to this work. Biomass detection by UV-Vis. Berbert-Molina et al. ([2008])
   0.36-2.66 g/L     Similar conditions of culture to this work. Farrera et al. ([1998])
    0.95    Similar conditions of culture to this work. Holmberg and Sievänen ([1980])
    0.53    Similar conditions of culture to this work. Rivera et al. ([1999])
  1. aMaximum specific growth rate (1/h).
  2. bYield of biomass from glucose (g biomass/g glucose).
  3. cYield of Cry from biomass (g Cry/g biomass). This value was calculated with the biomass and Cry protein concentration reported.
  4. dThis value was calculated considering that the weight of one B. thuringiensis cell is 2.3 pg (Rodriguez and de la Torre, 1996).
  5. ePHB percentage of dry cell weight.
  6. fUnpublished data.
  7. gYield of active biomass from limiting substrate.
  8. hThis value was calculated with the glucose concentration in the media and the yield of biomass from glucose reported by the author.
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