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Table 2 Apparent kinetic parameters for FBP and MgCl 2 at pH 8.0 a

From: Diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three Gram-negative bacteria

  kcat(s-1) vmin(s-1)b S0.5(mM) n H c
FBP     
FNLDH 45 (1) 35 (0.6) 4.2 (0.2) 1.7 (0.05)
PALDH 150 (30) 100 (2) 12 (3) 1.3 (0.07)
ECLDH 25 (1) 180 (2) 1.3 (0.01) 1.5 (0.2)
Mg2+     
FNLDH 22 (0.7) 37 (0.2) 1.5 (0.1) 1.3 (0.09)
PALDH 140 (2) 100 (1) 0.16 (0.008) 1.1 (0.08)
ECLDH N.D.d N.D. N.D.  
  1. aParameters were determined as described in the `Materials and methods' section, and standard deviations are shown in parentheses. bvmin is reaction velocity in the absence of FBP or Mg2+. cHill coefficients (nH) are shown only for the cases that exhibited significant cooperativity. dN.D., not determined.