| |
kcat(s-1)
|
S0.5(mM)
|
kcat/S0.5(s-1 mM-1)
|
Ki(mM)b
|
n
H
c
|
|---|
|
Glyoxylate
| | | | | |
|
FNLDH
|
11 (0.1)
|
18 (0.4)
|
0.70 (0.007)
| | |
|
PALDH
|
880 (60)
|
6.8 (0.6)
|
130 (5)
|
44 (8)
| |
|
ECLDH
|
100 (5)
|
20 (2)
|
5.3 (0.2)
| |
1.5 (0.09)
|
|
Pyruvate
| | | | | |
|
FNLDH
|
80 (0.4)
|
0.34 (0.007)
|
230 (4)
| | |
|
PALDH
|
400 (10)
|
0.10 (0.009)
|
4,000 (300)
|
1.8 (0.1)
| |
|
ECLDH
|
410 (8)
|
2.6 (0.09)
|
160 (2)
| |
1.4 (0.05)
|
|
2-Ketobutyrate
| | | | | |
|
FNLDH
|
140 (0.7)
|
0.31 (0.006)
|
440 (7)
| | |
|
PALDH
|
100 (0.6)
|
0.64 (0.01)
|
150 (3)
| | |
|
ECLDH
|
31 (2)
|
30 (4)
|
1.0 (0.05)
| |
1.4 (0.08)
|
|
2-Ketovalerate
| | | | | |
|
FNLDH
|
140 (0.5)
|
0.68 (0.01)
|
200 (3)
| | |
|
PALDH
|
69 (0.5)
|
4.0 (0.07)
|
18 (3)
| | |
|
ECLDH
|
N.D.d
|
N.D.
|
<0.01
| | |
|
2-Ketoisovalerate
| | | | | |
|
FNLDH
|
120 (0.7)
|
3.5 (0.07)
|
35 (0.5)
| | |
|
PALDH
|
N.D.
|
N.D.
|
<0.4
| | |
|
ECLDH
|
N.D.
|
N.D.
|
<0.01
| | |
|
2-Ketocaproate
| | | | | |
|
FNLDH
|
230 (1)
|
2.6 (0.06)
|
87 (1)
| | |
|
PALDH
|
160 (1)
|
5.8 (0.1)
|
27 (0.4)
| | |
|
ECLDH
|
N.D.
|
N.D.
|
<0.01
| | |
|
2-Ketoisocaproate
| | | | | |
|
FNLDH
|
200 (2)
|
3.8 (0.1)
|
53 (1)
| | |
|
PALDH
|
39 (0.3)
|
12 (0.2)
|
3.2 (0.03)
| | |
|
ECLDH
|
N.D.
|
N.D.
|
<0.01
| | |
|
Oxaloacetate
| | | | | |
|
FNLDH
|
80 (1)
|
2.5 (0.09)
|
31 (0.7)
|
140 (20)
| |
|
PALDH
|
410 (4)
|
1.7 (0.06)
|
230 (6)
| | |
|
ECLDH
|
1,100 (200)
|
54 (10)
|
21 (0.6)
|
5.0 (1)
| |
|
Hydroxypyruvate
| | | | | |
|
FNLDH
|
160 (2)
|
18 (0.4)
|
8.7 (0.07)
| | |
|
PALDH
|
420 (10)
|
3.0 (0.2)
|
140 (3)
|
19 (1)
| |
|
ECLDH
|
50 (0.5)
|
12 (0.3)
|
4.2 (0.06)
| | |
|
Phenylpyruvate
| | | | | |
|
FNLDH
|
120 (7)
|
11 (0.9)
|
11 (0.3)
|
23 (3)
| |
|
PALDH
|
18 (0.2)
|
7.1 (0.2)
|
2.6 (0.04)
| | |
|
ECLDH
|
N.D.
|
N.D.
|
<0.01
| | |
|
Hydroxyphenylpyruvate
| | | | | |
|
FNLDH
|
N.D.
|
N.D.
|
<0.02
| | |
|
PALDH
|
N.D.
|
N.D.
|
<0.4
| | |
|
ECLDH
|
N.D.
|
N.D.
|
<0.01
| | |
- aParameters were determined as described in the `Materials and methods' section, and standard deviations are shown in parentheses. bThe inhibition constants (Ki) and cHill coefficients (nH) are shown only for the cases in which significant substrate inhibition and cooperativity were observed, respectively. dN.D., not determined. The activities were too weak for determination of exact values.
