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Figure 6 | AMB Express

Figure 6

From: Diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three Gram-negative bacteria

Figure 6

Effects of intermediary metabolites and ions at pH 8.0. a) Effects of intermediary metabolites and ions on FNLDH (white boxes), PALDH (grey boxes), and ECLDH (black boxes). The activities were measured in 50 mM Bicine-NaOH buffer (pH 8.0) containing 0.1 mM NADH, 2.5 mM (for FNLDH), 1.2 mM (for PALDH), or 7.5 mM (for ECLDH) pyruvate, and 1 mM indicated effectors. b-d) the saturation curves for FBP or MgCl2. The reaction velocities for FNLDH (b), PALDH (c), and ECLDH (d) were measured in 50 mM Bicine-NaOH buffer (pH 8.0) containing 0.1 mM NADH, 2.5 mM (for FNLDH), 1.2 mM (for PALDH), or 7.5 mM (for ECLDH) pyruvate, and the indicated concentrations of FBP (open circles) or MgCl2 (closed circles). The lines indicate the calculated saturation curves obtained with apparent kinetic parameters (Table 2). The data of MgCl2 saturation curve of ECLDH was not fitted with any equations used in this study. e-g) the saturation curves for pyruvate with or without FBP or MgCl2. The reaction velocities for FNLDH (e), PALDH (f), and ECLDH (g) were measured in 50 mM Bicine-NaOH buffer (pH 8.0) containing 0.1 mM NADH, the indicated concentrations of pyruvate with no effector (white circles), 10 mM (for FNLDH and PALDH) or 5 mM (for ECLDH) FBP (grey circles), and 5 mM (for FNLDH and PALDH) or 2.4 mM (for ECLDH) MgCl2 (black circles). The lines indicate the calculated saturation curves obtained with kinetic parameters (Table 3).

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