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Figure 2 | AMB Express

Figure 2

From: Diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three Gram-negative bacteria

Figure 2

pH-stability of FNLDH (a), PALDH (b), and ECLDH (c), and heat-stability of the enzymes (d). a-c) Each enzyme was treated at 30°C for 1 h in sodium citrate (open circles), sodium acetate (closed circles), MES-NaOH (open triangles), MOPS-NaOH (closed triangles), HEPES-NaOH (open squares), Bicine-NaOH (N,N-Bis(2-hydroxyethyl)glycine) (closed squares), CHES-NaOH (N-cyclohexyl-2-aminoethanesulfonic acid) (open diamonds), and CAPS-NaOH (N-cyclohexyl-3-aminopropanesulfonic acid) (closed diamonds) buffers. d) FNLDH (white circles and solid lines), PALDH (grey circles and dashed lines), and ECLDH (black circles and dotted lines) were treated at various temperatures for 30 min in the buffers described in `Materials and methods'.

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