Table 2 Modifications made to standard Kirk’s medium

Low-N Kirk’s (1.2 mm)

Basic Kirk's containing 0.22 g L^-1 ammonium tartrate.

High-N Kirk’s (12 mm)

Basic Kirk's containing 2.2 g L^-1 ammonium tartrate.

Low-Glu Kirk's

Basic Kirk's containing 1 g L^-1 glucose.

Low-N/Low-Glu Kirk's

Basic Kirk's containing 1 g L^-1 glucose & 0.22 g L^-1 ammonium tartrate.

Low-N Kirk's + 200 μM Mn(II)

Basic Kirk's containing 0.22 g L^-1 ammonium tartrate & 0.034 g L^-1 MnSO₄.H₂O.