Table 1 Bacterial strains and plasmids used in this study

Rhodococcus opacus R7

Long-medium-chain n-alkane degrader, alkB⁺, nar⁺, gen⁺, o-xyl⁺

Di Gennaro et al. [2001]; Di Gennaro et al. [2010]

Rhodococcus erythropolis AP

Diesel Fuel degrader

Maffei [2004]

Escherichia coli DH5α

dlac ZΔM15, rec A1, end A1, gyr A96, thi-1, hsd R17(rK⁻, mK⁺), sup E44, rel A1, deo R, Δ(lac ZYA-arg F)U169

Promega

Escherichia coli DH5α (pDrive-alk R7)

E. coli DH5α containing the cloning vector pDrive, alkB fragment, Amp^r

This study

Escherichia coli DH5α (pTipQT1-alk R7)

E. coli DH5α containing the recombinant expression vector pTipQT1, alkB fragment, Thio^r, Amp^r

This study

Rhodococcus erythropolis AP (pTipQT1-alk R7)

R. erythropolis AP containing the recombinant expression vector pTipQT1, alkB fragment, Thio^r, Tc^r

This study

plasmids

pDrive

E. coli cloning vector, Amp^r

Qiagen

pDrive-alk R7

pDrive containing alkB fragment from R. opacus R7

This study

pTipQT1

Shuttle-vector Amp^rE. coli – Tc^rRhodococcus spp.

Nakashima and Tamura [2004b]

pTipQT1-alk R7

pTipQT1 containing alkB fragment from R. opacus R7

This study