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Table 1 Bacterial strains and plasmids used in this study

From: Biodegradation of variable-chain-length n-alkanes in Rhodococcus opacus R7 and the involvement of an alkane hydroxylase system in the metabolism

Strain or plasmid Description Reference or source
Strains   
Rhodococcus opacus R7 Long-medium-chain n-alkane degrader, alkB+, nar+, gen+, o-xyl+ Di Gennaro et al. [2001]; Di Gennaro et al. [2010]
Rhodococcus erythropolis AP Diesel Fuel degrader Maffei [2004]
Escherichia coli DH5α dlac ZΔM15, rec A1, end A1, gyr A96, thi-1, hsd R17(rK, mK+), sup E44, rel A1, deo R, Δ(lac ZYA-arg F)U169 Promega
Escherichia coli DH5α (pDrive-alk R7) E. coli DH5α containing the cloning vector pDrive, alkB fragment, Ampr This study
Escherichia coli DH5α (pTipQT1-alk R7) E. coli DH5α containing the recombinant expression vector pTipQT1, alkB fragment, Thior, Ampr This study
Rhodococcus erythropolis AP (pTipQT1-alk R7) R. erythropolis AP containing the recombinant expression vector pTipQT1, alkB fragment, Thior, Tcr This study
plasmids   
pDrive E. coli cloning vector, Ampr Qiagen
pDrive-alk R7 pDrive containing alkB fragment from R. opacus R7 This study
pTipQT1 Shuttle-vector AmprE. coli – TcrRhodococcus spp. Nakashima and Tamura [2004b]
pTipQT1-alk R7 pTipQT1 containing alkB fragment from R. opacus R7 This study