Degradation of β-HCH in reaction mixtures containing LinB
and its mutant derivatives. LinBUT wild-type (a) and its mutants, M1-1 (b), M1-2 (c), M1-3 (d), M1-4 (e), M1-5 (f), M1-6 (g), M1-7 (h), M2-1 (i), M3-1 (j), M3-2 (k), M3-3 (l). The closed circle and closed and open triangles represent β-HCH, PCHL, and TCDL, respectively. Each value given is the mean of triplicates. Kinetic data were fitted to the irreversible two-step reaction scheme of β-HCH conversion to TCDL via PCHL (Scheme 1 in Materials and methods) by using GEPASI 3.2 software (Mendes ) and shown in solid lines. The specificity constants and their standard errors for both reaction steps (k1 and k2) were obtained from the calculation (Table 1).