Dot plots of DNA content (DAPI, in arbitrary fluorescence units (A.F.U.)) versus forward scatter (FSC, in A.F.U.) at different growth rates 0.1 h−1, 0.2 h−1and 0.7 h−1. The dataset of the biological replicate can be found in the Additional file 2: Figure S1. Cells of P. putida KT2440 grown at steady state conditions in chemostats were stained with DAPI and analyzed by flow cytometry. The DNA content and the forward scatter increased with increasing growth rate. The indicated gates (C1, C2, Cx) were used for sorting 5x106 cells per subpopulation for further mass spectrometric analysis.