Table 1 Bacterial strains, plasmids and oligonucleotides used in this study
Description | Reference or source | |
|---|---|---|
Strains | ||
Ralstonia eutropha H16 | Wild type | DSM 428 |
Escherichia coli | ||
BL21 (DE3) | F−omp T hsd SB (rB−, mB−) gal dcm (DE3) | Novagen |
Plasmids | ||
pCOLADuet-1::3HAD | pCOLADuet-1 with fadB’ as Bam HI/Hin dIII fragment | Volodina et al. [2014] |
pET-19b::pct | pET-19b with pct as Nde I/Xho I fragment | Lindenkamp et al. [2013] |
pET-23a | E. coli expression vector (Apr, T7 promoter) | Novagen |
pET-23a::bktB | pET-23a with bktB as Nde I/Hin dIII fragment | This study |
Oligonucleotides | ||
Forward primer BktB Nde I* | AAACATATG ACGCGTGAAGTGGTAGTGGTAAGC | This study |
Reverse primer BktB Hind III* | AAAAAGCTT GATACGCTCGAAGATGGCGGC | This study |