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Figure 3 | AMB Express

Figure 3

From: (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (FadB’) from fatty acid degradation operon of Ralstonia eutropha H16

Figure 3

Dependence of the inhibition of FadB’ activity on the concentration of acetyl-CoA, propionyl-CoA and CoA. The dotted lines demonstrate the inhibitory effect on FadB’ activity measured with acetoacetyl-CoA as substrate. The reaction mixture contained in 100 mM Tris–HCl buffer (pH 7.0) 0.1 mM acetoacetyl-CoA, 0.2 mM NADH, 0.3 μg FadB’ Re and the inhibitor CoA or acetyl-CoA at the concentrations as indicated. The straight lines demonstrate the inhibitory effect on FadB’ measured with crotonyl-CoA as substrate. The reaction mixture contained in 100 mM Tris–HCl (pH 8.1) 0.1 mM crotonyl-CoA, 2 mM pyruvate, lactate dehydrogenase (9 U), 1.5 mM NAD+, 25 mM MgCl2 and the inhibitor CoA, acetyl-CoA or propionyl-CoA at the concentrations as indicated. All values are presented as mean values of at least two measurements, standard deviations varied between 0.01% and 3.58%.

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