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Table 1 Kinetic analysis of synthetic and natural substrates determined in 50 mM sodium phosphate buffer (pH 6.0) at 50°C

From: Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris

Substrate Km(mM) Vmax(μmol min−1 mg−1) kcat(sec−1) kcat/Km (mM−1 s−1)
pNPX 1 ± 0.3 250 ± 0.001 352.7 352.7
pNPX + 20 mM Xylose 2.9 ± 0.5 250.5 ± 23 353.4 121.9
pNPX + 20 mM Fructose 0.1 ± 0.06 14.5 ± 3 20.6 206
Xylobiose (X2) 2.6 ± 0.3 25.5 ± 0.1 36.0 13.8
Xylotriose (X3) 3.07 ± 0.3 21.3 ± 0.3 30.1 9.7
Xylotetraose (X4) 0.62 ± 0.4 14.5 ± 0.003 20.5 33.1
  1. ± standard deviation of the means of triplicate data.