Table 1 Kinetic analysis of synthetic and natural substrates determined in 50 mM sodium phosphate buffer (pH 6.0) at 50°C
Substrate | Km(mM) | Vmax(μmol min−1 mg−1) | kcat(sec−1) | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|
pNPX | 1 ± 0.3 | 250 ± 0.001 | 352.7 | 352.7 |
pNPX + 20 mM Xylose | 2.9 ± 0.5 | 250.5 ± 23 | 353.4 | 121.9 |
pNPX + 20 mM Fructose | 0.1 ± 0.06 | 14.5 ± 3 | 20.6 | 206 |
Xylobiose (X2) | 2.6 ± 0.3 | 25.5 ± 0.1 | 36.0 | 13.8 |
Xylotriose (X3) | 3.07 ± 0.3 | 21.3 ± 0.3 | 30.1 | 9.7 |
Xylotetraose (X4) | 0.62 ± 0.4 | 14.5 ± 0.003 | 20.5 | 33.1 |