Table 2 Primers used in vector construction
Primer | Sequence |
|---|---|
JS_gatC _FW | 5’-GAGAATGCATATGTCTGAGCCTAAGAACCAGC-3’ |
JS_gatC _RV | 5’-GAGAGCGGCCGCTCATATTTTGCTCGTATTCC-3’ |
JS_gatC mut2_FW | 5’-GTTGCCAAAGTGGGAGACCTGCAAACCCTGGGTC-3’ |
JS_gatC mut2_RV | 5’-GACCCAGGGTTTGCAGGTCTCCCACTTTGGCAAC-3’ |
JS_XlnDp_FW | 5’-AGTCAATCGGTCATTCTCCG-3’ |
JS_gatA _RV | 5’-CTCTTGGAGGACGCAAAACC-3’ |
JS_gatB _RV | 5’-CATGGAAGCGGTGATCTAGG-3’ |