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Table 2 The average Z-scores for 1D comparative LC/MS proteomic analyses (four columns after description) and MRM results (last four columns) for the key proteins

From: Reduced catabolic protein expression in Clostridium butyricum DSM 10702 correlate with reduced 1,3-propanediol synthesis at high glycerol loading

Locus Tag Description ST170 mM- EXP170 mM ST620 mM-EXP620 mM EXP620 mM-EXP170 mM ST620 mM-ST170 mM ST170 mM- EXP170 mM ST620 mM- EXP620 mM EXP620 mM-EXP170 mM ST620 mM-ST170 mM
CBY_0205 phosphate acetyltransferase −0.04 1.24 −0.85 0.25 NQ 0.68 NQ NQ
CBY_0206 acetate kinase 0.00 0.19 −1.00 −0.99 0.25 0.60 −0.73 −0.40
CBY_0233 pyruvate:ferredoxin oxidoreductase −0.84 0.09 0.30 1.32 NQ NQ NQ NQ
CBY_0500 1.3-propanediol dehydrogenase −0.26 −0.47 0.06 −0.12 −0.20 −0.32 −0.61 −0.75
CBY_0501 glycerol dehydratase (activator) −0.63 −0.52 −1.02 −1.05 −0.64 −0.59 −1.56 −1.53
CBY_0502 glycerol dehydratasea −0.34 −0.87 0.24 −0.21 −0.71 −0.74 −0.63 −0.67
CBY_0505 dihydroxyacetone kinase. (phosphotransfer subunit) −0.31 −1.55 −1.14 −2.52 0.18 −0.06 −1.32 −1.57
CBY_0506 dihydroxyacetone kinase (L subunit) 0.47 0.28 0.08 −0.15 0.52 −0.27 −0.15 −0.95
CBY_0508 Glycerol dehydrogenaseb 0.07 −0.19 0.04 −0.24 −0.39 −0.17 −1.97 −1.77
CBY_0742 L-lactate dehydrogenase 0.19 −0.79 1.08 0.29 0.22 −0.59 0.26 −0.56
CBY_1290 acetyl-CoA acetyltransferase 0.58 0.06 1.19 0.83 0.23 0.27 0.68 0.71
CBY_1889 pyruvate:ferredoxin oxidoreductase −0.35 0.00 0.32 0.75 −0.04 0.50 −1.12 −0.60
CBY_2300 FeFe hydrogenase-1 −0.27 −1.46 1.57 0.69 −0.27 −0.49 −0.43 −0.67
CBY_2341 L-lactate dehydrogenase 0.66 0.10 0.51 −0.02 1.31 0.95 0.13 −0.25
CBY_2757 L-lactate dehydrogenase −1.08 −1.43 0.28 0.08 NQ 0.07 NQ NQ
CBY_2919 phosphate butyryltransferase 0.60 0.16 1.15 0.86 0.36 0.59 0.00 0.22
CBY_2920 butyrate kinase 0.62 0.54 0.89 0.91 0.26 0.32 −0.38 −0.33
CBY_3041 3-hydroxybutyryl-CoA dehydratase 0.50 0.29 1.17 1.11 NQ NQ NQ NQ
CBY_3042 acyl-coa dehydrogenase (short-chain specific) 0.20 −0.25 0.90 0.60 −0.06 −0.06 0.96 0.94
CBY_3045 3-hydroxybutyryl-coa dehydrogenase 0.24 0.20 1.16 1.28 0.31 0.06 −0.04 −0.30
CBY_3235 Glycerol dehydrogenasec −0.02 −0.52 −1.47 −2.23 −0.34 −0.36 −2.66 −2.70
CBY_3258 acyl-coa dehydrogenase (short-chain specific) 0.13 0.41 −0.39 −0.20 0.16 0.64 −1.51 −1.05
CBY_3642 pyruvate:ferredoxin oxidoreductase −0.05 0.29 0.02 0.35 −0.01 0.49 −1.04 −0.56
CBY_3690 dihydroxyacetone kinase.(L subunit) 0.57 0.75 −1.75 −1.94 0.13 0.40 −2.44 −2.19
CBY_3691 dihydroxyacetone kinase (DhaK subunit) 0.42 0.58 −0.79 −0.81 NQ NQ NQ NQ
CBY_3747 NADPH-dependent butanol dehydrogenase 0.35 −1.12 −0.12 −1.61 −0.13 −0.22 −1.01 −1.11
CBY_3751 NADPH-dependent butanol dehydrogenase 0.23 0.08 0.36 0.26 0.53 0.18 −0.57 −0.94
CBY_3753 Ethanol dehydrogenase (aldehyde-alcohol dehydrogenase 2) −2.58 −1.08 2.61 4.78 −0.27 −0.12 −0.15 −0.01
CBY_9999 glycerol uptake facilitator protein NQ NQ NQ NQ NQ NQ NQ NQ
  1. NQ: Not quantifiable. aWrongly annotated as pyruvate formate-lyase.bWrongly annotated as 3-dehydroquinate synthase.cWrongly annotated as 3-dehydroquinate synthase.
  2. The values indicate the differences between the samples taken from different growth phases (EXP; exponential and ST; stationary) and/or different initial glycerol concentrations (170 mM and 620 mM) of C. butyricum cells. Numbers in bold indicate differences in protein measurements that are greater that 2 fold.