Table 2 Relative changes in protein expression between S. cerevisiae wild type and engineered strains

GAL1

Galactokinase

9

0.870 ± 0.340

0.587 ± 0.225

3.783 ± 0.215

GAL7

Galactose-1-phosphate uridylyltransferase

2

1.230 ± 0.005

1.387 ± 0.965

1.008 ± 0.021

HXK1

Hexokinase-1

2

0.723 ± 0.259

0.691 ± 0.104

3.489 ± 0.368

PGI1

Glucose-6-phosphate isomerase

3

1.032 ± 0.460

1.330 ± 0.180

9.891 ± 0.251

PFK2

Phosphofructokinase

3

1.236 ± 0.155

1.149 ± 0.100

1.426 ± 0.197

FBA1

Fructose-biophosphate aldolase

8

1.094 ± 0.155

0.871 ± 0.235

2.304 ± 0.942

TPI1

Triosephosphate isomerase

6

1.123 ± 0.380

0.849 ± 0.070

1.038 ± 0.357

TDH

Glyceraldehyde 3-phosphate dehydrogenase

15

1.117 ± 0.305

0.925 ± 0.220

1.664 ± 0.541

PGK1

Phosphoglycerate kinase

19

1.066 ± 0.225

0.798 ± 0.120

1.286 ± 0.076

GPM1

Phosphoglycerate mutase 1

14

1.047 ± 0.210

0.750 ± 0.145

1.500 ± 0.457

ENO

Enolase

19

1.185 ± 0.160

0.912 ± 0.155

2.176 ± 0.478

PYK1

Pyruvate kinase

6

1.186 ± 0.200

0.831 ± 0.195

1.831 ± 0.147

CIT1

Citrate synthase, mitochondrial

3

1.256 ± 0.820

1.982 ± 0.230

5.580 ± 0.248

ACO1

Aconitate hydratase, mitochondrial

3

0.961 ± 0.400

1.033 ± 0.075

1.737 ± 0.128

ATP1

ATP synthase subunit alpha, mitochondrial

6

1.222 ± 0.260

1.005 ± 0.030

1.157 ± 0.160

ATP2

ATP synthase subunit beta, mitochondrial

8

1.176 ± 0.040

1.123 ± 0.085

3.216 ± 0.205

LEU1

3-isopropylmalate dehydratase

3

0.883 ± 0.075

1.023 ± 0.335

1.424 ± 0.200

LEU2

3-isopropylmalate dehydrogenase

17

3.477 ± 0.630

1.070 ± 0.335

2.570 ± 0.254

MET6

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

10

1.050 ± 0.125

0.881 ± 0.430

2.018 ± 0.121

PDC

Pyruvate decarboxylase isozyme

12

1.305 ± 0.355

1.118 ± 0.115

1.894 ± 0.218

TIF

ATP-dependent RNA helicase eIF4A

3

1.408 ± 0.025

0.752 ± 0.295

1.655 ± 0.245

TEF1

Elongation factor 1-alpha

8

0.910 ± 0.375

0.758 ± 0.185

1.507 ± 0.110

RPL4

60s ribosomal protein L4

9

1.245 ± 0.255

0.778 ± 0.035

1.418 ± 0.068

RPL19

60s ribosomal protein L19

2

1.218 ± 0.285

1.114 ± 0.805

2.995 ± 0.197

HSP 12

12 kDa Heat shock protein

2

2.199 ± 0.640

0.882 ± 0.135

2.308 ± 0.219

HSP 26

Heat shock protein 26

3

2.281 ± 0.675

1.823 ± 0.360

2.453 ± 0.195

STI1

Heat shock protein STI1

2

1.363 ± 0.665

0.485 ± 0.035

3.450 ± 0.377

POR1

Mitochondrial outer membrane protein porin 1

4

1.033 ± 0.395

0.808 ± 0.445

2.785 ± 0.066

SAM2

S-adenosylmethionine synthetase 2

2

1.271 ± 0.300

0.624 ± 0.085

2.125 ± 0.151

YMR226C

Uncharacterized oxidoreductase YMR226C

2

1.856 ± 0.375

1.260 ± 0.110

3.051 ± 0.265

SOD1

Superoxide dismutase [Cu-Zn]

2

6.360 ± 0.420

3.942 ± 1.400

7.910 ± 0.330