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Table 3 Kinetic parameters of the wild-type and mutant enzymes 1

From: Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1

Enzyme kcat(s−1) Km(mM) kcat/Km(mM−1 s−1)
Wild-type 91.6 ± 2.75 0.79 ± 0.04 116
D17N 110 ± 4.48 0.98 ± 0.05 113
D212N 75.2 ± 3.13 1.10 ± 0.10   68.6
E237Q 96.1 ± 0.98 0.91 ± 0.03 105
D58N 68.5 ± 1.37 0.99 ± 0.07   70.0
D138N 59.5 ± 6.14 0.77 ± 0.01   77.3
D183N 53.0 ± 3.03 0.74 ± 0.05   71.6
G61D 47.2 ± 1.77 0.82 ± 0.13   63.6
G262D 61.3 ± 1.86 0.93 ± 0.15   66.2
S277D 73.6 ± 5.51 0.80 ± 0.12   92.5
  1. 1Parameters were determined at 40°C with N-suc-AAPF-pNA as a substrate. The experiments were performed in either duplicate or triplicate.