AMB Express

Table 3 Kinetic parameters of the wild-type and mutant enzymes ¹

From: Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1

Enzyme	k_cat(s⁻¹)	K_m(mM)	k_cat/K_m(mM⁻¹ s⁻¹)
Wild-type	91.6 ± 2.75	0.79 ± 0.04	116
D17N	110 ± 4.48	0.98 ± 0.05	113
D212N	75.2 ± 3.13	1.10 ± 0.10	68.6
E237Q	96.1 ± 0.98	0.91 ± 0.03	105
D58N	68.5 ± 1.37	0.99 ± 0.07	70.0
D138N	59.5 ± 6.14	0.77 ± 0.01	77.3
D183N	53.0 ± 3.03	0.74 ± 0.05	71.6
G61D	47.2 ± 1.77	0.82 ± 0.13	63.6
G262D	61.3 ± 1.86	0.93 ± 0.15	66.2
S277D	73.6 ± 5.51	0.80 ± 0.12	92.5

¹Parameters were determined at 40°C with N-suc-AAPF-pNA as a substrate. The experiments were performed in either duplicate or triplicate.

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