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Table 2 Oligonucleotide primers used for site-directed mutagenesis

From: Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1

Name Oligonucleotide sequence Mutant name
AQN-D17N 5'-CCAGCGGA ACCTTCCC-3' D17N
AQN-D58N 5'-GGTAGGCTATA ACGCCTTAGGG-3' D58N
AQN-G61D 5'-ACGCCTTAGAT GGGAACG-3' G61D
AQN-D138N 5'-CTGCCCTGA ACAACGCC-3' D138N
AQN-D183N 5'-ATCTTCCA ACGCCCGTG-3' D183N
AQN-D212N 5'-ACACCTCGA ACACGGCC-3' D212N
AQN-E237Q 5'-CTTTGTATCTAC AGCAAAATCTTC-3' E237Q
AQN-G262D 5'-GCTTTCGGA TATCGGATCG-3' G262D
AQN-S277D 5'-CCTGCTCGAT TCGGGGAG-3' S277D
  1. Mismatched bases are shown in italic.