Table 2 Oligonucleotide primers used for site-directed mutagenesis
Name | Oligonucleotide sequence | Mutant name |
|---|---|---|
AQN-D17N | 5'-CCAGCGGA ACCTTCCC-3' | D17N |
AQN-D58N | 5'-GGTAGGCTATA ACGCCTTAGGG-3' | D58N |
AQN-G61D | 5'-ACGCCTTAGAT GGGAACG-3' | G61D |
AQN-D138N | 5'-CTGCCCTGA ACAACGCC-3' | D138N |
AQN-D183N | 5'-ATCTTCCA ACGCCCGTG-3' | D183N |
AQN-D212N | 5'-ACACCTCGA ACACGGCC-3' | D212N |
AQN-E237Q | 5'-CTTTGTATCTAC AGCAAAATCTTC-3' | E237Q |
AQN-G262D | 5'-GCTTTCGGA TATCGGATCG-3' | G262D |
AQN-S277D | 5'-CCTGCTCGAT TCGGGGAG-3' | S277D |