Figure 3From: Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1Temperature-dependence of the activity of the wild-type and mutant enzymes. The temperature-dependence of the activity was measured as described in the Materials and methods section. (a) mutants lacking an intrinsic salt bridge, (b) mutants lacking a common salt bridge, (c) mutants in which a salt bridge may have been introduced.Back to article page