Plasmids | Relevant features | Origin |
---|---|---|
p426GPD | Multicopy URA3 2 μm TDH3 p-CYC1 t | (Mumberg et al. [1995]) |
p426GPD –YAP1 | TDH3 p-YAP1-CYC1 t | This work |
pUG6 | kanMX flanked by loxP sites | (Güldener et al. [1996]) (EUROSCARF; accession number P30114) |
YE-plac 112 KanR | Multicopy, KanMX | (Jeppsson et al. [2003]) |
YE-plac 112 KanR-YAP1 | TDH3 p-YAP1-CYC1 t | This work |
pJET1,2 | Multicopy | Thermo Scientific, Belgium |
pJET1,2-attB-KanMX-attP | Multicopy, KanMX under TEFp | This work |
p-intYAP1 | TDH3 p-YAP1-CYC1 t – KanMX attB/attP system; integrative | This work |
pSTBlue-1 | multi-purpose cloning vector with dual kanamycin/ampicillin resistance. | Novagen (EMD Millipore) |
pSTBlue-YLR446W | pSTBlue-1 with the YLR446W gene cloned into the multiple cloning region | This work |
pYX012 | Integrative; URA3; TPI1p | R&D System, Inc., Wiesbaden, D |
pYX012-LoxPkanMXLoxP | pYX012, with the KanMX cassette flanked by loxP sites, deriving from pUG6 | This work |
pYX012-LoxPKanMXLoxP-MCR1 | pYX012- LoxPKanMXLoxP with the MCR1 gene inserted in the MCS under the control of the TPI1 promoter | This work |
pSTBlue-YLR446WΔ- LoxPKanMXLoxP-MCR1 | pSTBlue-YLR446W with the LoxPKanMXLoxP-MCR1 cassette, deriving from pYX012 LoxPKanMXLoxP-MCR1, inserted into the YLR446W sequence | This work |
pSH65 | Centromeric plasmid, GAL1p-cre, bler | (Gueldener et al. [2002]) (EUROSCARF; accession number P30122) |
pJET1,2- attB-hph- attP | Multicopy, hph under TEFp | This work |
pBEVY-Nat-phiC31 | Multicopy, PhiC31 integrase | This work |