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Table 2 Plasmids used in the current work

From: Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate

Plasmids

Relevant features

Origin

p426GPD

Multicopy URA3 2 μm TDH3 p-CYC1 t

(Mumberg et al. [1995])

p426GPD –YAP1

TDH3 p-YAP1-CYC1 t

This work

pUG6

kanMX flanked by loxP sites

(Güldener et al. [1996]) (EUROSCARF; accession number P30114)

YE-plac 112 KanR

Multicopy, KanMX

(Jeppsson et al. [2003])

YE-plac 112 KanR-YAP1

TDH3 p-YAP1-CYC1 t

This work

pJET1,2

Multicopy

Thermo Scientific, Belgium

pJET1,2-attB-KanMX-attP

Multicopy, KanMX under TEFp

This work

p-intYAP1

TDH3 p-YAP1-CYC1 t – KanMX attB/attP system; integrative

This work

pSTBlue-1

multi-purpose cloning vector with dual kanamycin/ampicillin resistance.

Novagen (EMD Millipore)

pSTBlue-YLR446W

pSTBlue-1 with the YLR446W gene cloned into the multiple cloning region

This work

pYX012

Integrative; URA3; TPI1p

R&D System, Inc., Wiesbaden, D

pYX012-LoxPkanMXLoxP

pYX012, with the KanMX cassette flanked by loxP sites, deriving from pUG6

This work

pYX012-LoxPKanMXLoxP-MCR1

pYX012- LoxPKanMXLoxP with the MCR1 gene inserted in the MCS under the control of the TPI1 promoter

This work

pSTBlue-YLR446WΔ- LoxPKanMXLoxP-MCR1

pSTBlue-YLR446W with the LoxPKanMXLoxP-MCR1 cassette, deriving from pYX012 LoxPKanMXLoxP-MCR1, inserted into the YLR446W sequence

This work

pSH65

Centromeric plasmid, GAL1p-cre, bler

(Gueldener et al. [2002]) (EUROSCARF; accession number P30122)

pJET1,2- attB-hph- attP

Multicopy, hph under TEFp

This work

pBEVY-Nat-phiC31

Multicopy, PhiC31 integrase

This work