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Table 2 Degradative actions of microbial and commercial xylanolytic activities on A. donax biomass and transglycosylation reactions

From: Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax

  Biocatalyst S/B RS% Xyl% Glc% Useful results
1 TL-xylanase 3.3 23.5 2.26 8.6 xylobiose 3.3 g/L
pentasaccharide 1 (Figure 2), 2 g/L
2 TM-xylanase 2.6 38 0 0 -
3 CFE-TT 3.5 90 19.2 16.9 -
4 CFE-TT 1.2 100 28.6 6.8 -
5 CFE-TT 3.5 20 8.7 7.8 tetrasaccharide 2 (Figure 2) 2.64 g/L
6 CFE-TN 1.2 75 40.5 15.2 see Figure 3
7 ES-GTM1b 13.5 73.5 26.5 0 see Figure 3
8 ES-GTM1a 36.3 63.1 9.85 0 see Figure 3
    Comment
9 TM-xylanase 7.7 Formation of mono and dixylosylated acceptor
10 CFE-TN 1.2 Formation of mono and dixylosylated acceptor
11 ES-GTM1 13.5 Formation of mono, di and trixylosylated acceptor
12 CFE-TT 3.5 Formation of mono and dixylosylated acceptor
  1. Data are expressed in percentages using means of the values obtained by chemical and enzymatic determinations conducted in triplicate that did not differ by more than 5%. S/B = amount of biocatalyst, mg substrate/mg total protein; RS = Reducing sugars yield % (DNS); TL = Thermomyces lanuginosus xylanase; TM = Thermotoga maritima xylanase; CFE-TT = cell free extract of Thermoanaeorbacterium thermostercoris; CFE-TN = cell free extract of T. neapolitana; ES-GTM1 = extracellular suspension Geobacillus thermantarcticus.