Table 7 ANOVA for response surface quadratic model of the complex and defined media

Complex medium

Model

33907.83

14

2421.99

44.71

< 0.0001

Significant

A-Sucrose

345.73

1

345.73

6.38

0.0233

B-NaNO₃

1359.75

1

1359.75

25.1

0.0002

C-Yeast extract

675.49

1

675.49

12.47

0.003

D-K₂HPO₄

7094.29

1

7094.29

130.97

< 0.0001

AB

1015

1

1015

18.74

0.0006

AC

52.56

1

52.56

0.97

0.3402

AD

530.92

1

530.92

9.8

0.0069

BC

2264.16

1

2264.16

41.8

< 0.0001

BD

1.27

1

1.27

0.023

0.8806

CD

606.84

1

606.84

11.2

0.0044

A²

6695.69

1

6695.69

123.61

< 0.0001

B²

10541.5

1

10541.5

194.61

< 0.0001

C²

5955.23

1

5955.23

109.94

< 0.0001

D²

4934.49

1

4934.49

91.1

< 0.0001

Residual

812.53

15

54.17

Lack of Fit

645.21

10

64.52

1.93

0.2428

Not significant

Pure Error

167.32

5

33.46

Cor Total

34720.36

29

Defined medium

Model

61950.92

14

4425.07

23.27

< 0.0001

Significant

A-NaNO₃

1466.37

1

1466.37

7.71

0.0141

B-K₂HPO₄

7429.48

1

7429.48

39.07

< 0.0001

C-KH₂PO₄

1619.47

1

1619.47

8.52

0.0106

D-Trace elements

3979

1

3979

20.92

0.0004

AB

240.85

1

240.85

1.27

0.2781

AC

3034.41

1

3034.41

15.96

0.0012

AD

12478.18

1

12478.18

65.61

< 0.0001

BC

1745.83

1

1745.83

9.18

0.0084

BD

1394.94

1

1394.94

7.33

0.0162

CD

1498.72

1

1498.72

7.88

0.0133

A²

4217.35

1

4217.35

22.18

0.0003

B²

11914.43

1

11914.43

62.65

< 0.0001

C²

42.42

1

42.42

0.22

0.6435

D²

15571

1

15571

81.88

< 0.0001

Residual

2852.69

15

190.18

Lack of Fit

2166.36

10

216.64

1.58

0.3207

Not significant

Pure Error

686.33

5

137.27

Cor Total

64803.62

29