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Table 7 ANOVA for response surface quadratic model of the complex and defined media

From: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae

  Sum of   Mean F p-value  
Source Squares df Square Value Prob > F  
Complex medium       
Model 33907.83 14 2421.99 44.71 < 0.0001 Significant
A-Sucrose 345.73 1 345.73 6.38 0.0233  
B-NaNO3 1359.75 1 1359.75 25.1 0.0002  
C-Yeast extract 675.49 1 675.49 12.47 0.003  
D-K2HPO4 7094.29 1 7094.29 130.97 < 0.0001  
AB 1015 1 1015 18.74 0.0006  
AC 52.56 1 52.56 0.97 0.3402  
AD 530.92 1 530.92 9.8 0.0069  
BC 2264.16 1 2264.16 41.8 < 0.0001  
BD 1.27 1 1.27 0.023 0.8806  
CD 606.84 1 606.84 11.2 0.0044  
A2 6695.69 1 6695.69 123.61 < 0.0001  
B2 10541.5 1 10541.5 194.61 < 0.0001  
C2 5955.23 1 5955.23 109.94 < 0.0001  
D2 4934.49 1 4934.49 91.1 < 0.0001  
Residual 812.53 15 54.17    
Lack of Fit 645.21 10 64.52 1.93 0.2428 Not significant
Pure Error 167.32 5 33.46    
Cor Total 34720.36 29     
Defined medium       
Model 61950.92 14 4425.07 23.27 < 0.0001 Significant
A-NaNO3 1466.37 1 1466.37 7.71 0.0141  
B-K2HPO4 7429.48 1 7429.48 39.07 < 0.0001  
C-KH2PO4 1619.47 1 1619.47 8.52 0.0106  
D-Trace elements 3979 1 3979 20.92 0.0004  
AB 240.85 1 240.85 1.27 0.2781  
AC 3034.41 1 3034.41 15.96 0.0012  
AD 12478.18 1 12478.18 65.61 < 0.0001  
BC 1745.83 1 1745.83 9.18 0.0084  
BD 1394.94 1 1394.94 7.33 0.0162  
CD 1498.72 1 1498.72 7.88 0.0133  
A2 4217.35 1 4217.35 22.18 0.0003  
B2 11914.43 1 11914.43 62.65 < 0.0001  
C2 42.42 1 42.42 0.22 0.6435  
D2 15571 1 15571 81.88 < 0.0001  
Residual 2852.69 15 190.18    
Lack of Fit 2166.36 10 216.64 1.58 0.3207 Not significant
Pure Error 686.33 5 137.27    
Cor Total 64803.62 29     
  1. Note: For the complex medium: R2 = 0.9766, R2 (adj) = 0.9548, R2 (pred) = 0.8860, CV = 3.84%, Adeq Precision = 21.513.
  2. For the defined medium: R2 = 0.956, R2 (adj) = 0.9149, R2 (pred) = 0.7922, CV = 12.93%, Adeq Precision = 17.282.