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AMB Express

Table 6 Central composite design for the complex medium

From: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae

Run Coded factors AMVL (mg/L)
A B C D Actual Predicted
1 −1 −1 −1 −1 137.9 ± 5.3 129.1
2 1 −1 −1 −1 134.5 ± 6.2 132.3
3 −1 1 −1 −1 157.9 ± 7.1 152.1
4 1 1 −1 −1 183.0 ± 7.7 187.1
5 −1 −1 1 −1 176.8 ± 8.4 175.9
6 1 −1 1 −1 184.9 ± 8.0 179.1
7 −1 1 1 −1 154.0 ± 6.5 151.2
8 1 1 1 −1 189.8 ± 8.3 186.3
9 −1 −1 −1 1 195.0 ± 8.6 187.4
10 1 −1 −1 1 165.8 ± 7.5 167.5
11 −1 1 −1 1 211.6 ± 9.7 210.3
12 1 1 −1 1 224.1 ± 9.3 222.3
13 −1 −1 1 1 214.6 ± 9.1 209.5
14 1 −1 1 1 194.9 ± 6.8 189.6
15 −1 1 1 1 185.4 ± 5.7 184.8
16 1 1 1 1 195.2 ± 7.9 196.8
17 −2 0 0 0 161.0 ± 7.6 172.1
18 2 0 0 0 187.2 ± 6.4 187.3
19 0 −2 0 0 137.2 ± 4.1 148.7
20 0 2 0 0 179.2 ± 6.8 178.8
21 0 0 −2 0 167.3 ± 6.7 172.6
22 0 0 2 0 188.1 ± 7.3 193.9
23 0 0 0 −2 146.8 ± 5.2 154.2
24 0 0 0 2 219.2 ± 8.9 222.9
25 0 0 0 0 238.9 ± 9.5 242.2
26 0 0 0 0 248.8 ± 8.0 242.2
27 0 0 0 0 241.5 ± 8.2 242.2
28 0 0 0 0 243.3 ± 9.4 242.2
29 0 0 0 0 233.1 ± 6.7 242.2
30 0 0 0 0 247.5 ± 7.2 242.2
  1. Note: A, Sucrose; B, NaNO3; C, Yeast extract; D, K2HPO4.
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