Table 4 Statistical analysis of the Plackett-Burman design for the complex and defined media

Complex medium

constant

65.04

< 0.0001

A, Sucrose

22.1004

11.05

0.0201

B, NaNO₃

75.1828

37.59

< 0.0001

C, Yeast extract

30.4603

15.23

0.0025

D, KH₂PO₄

−4.7852

−2.39

0.5877

E, K₂HPO₄

27.8186

13.91

0.0049

F, KCl

8.7311

4.37

0.3272

G, MgSO₄.7H₂O

1.7222

0.86

0.8448

H, Trace elements

−15.9140

−7.96

0.0830

Defined medium

constant

42.14

< 0.0001

I, Sucrose

1.0233

0.51

0.7089

J, NaNO₃

27.1197

13.56

< 0.0001

K, K₂HPO₄

−8.5673

−4.28

0.0052

L, KH₂PO₄

−10.8947

−5.45

0.0008

M, KCl

−2.3609

−1.18

0.3930

N, MgSO₄.7H₂O

5.1297

2.56

0.0733

O, CaCl₂.2H₂O

−5.7316

−2.87

0.0542

P, Na₃C₆H₅O₇.2H₂O

2.7889

1.39

0.3149

Q, Biotin

1.1972

0.6

0.6625

R, Trace elements

−22.8260

−11.41

< 0.0001