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Table 3 Experimental design using the Plackett-Burman method with AMVL production for the defined medium

From: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae

Run Coded values AMVL (mg/L)
I J K L M N O P Q R
1 1 1 −1 1 1 1 −1 −1 −1 1 44.5 ± 2.2
2 −1 1 1 −1 1 1 1 −1 −1 −1 57.9 ± 3.1
3 1 −1 1 1 −1 1 1 1 −1 −1 32.6 ± 1.4
4 −1 1 −1 1 1 −1 1 1 1 −1 54.7 ± 2.6
5 −1 −1 1 −1 1 1 −1 1 1 1 17.7 ± 0.6
6 −1 −1 −1 1 −1 1 1 −1 1 1 11.7 ± 0.4
7 1 −1 −1 −1 1 −1 1 1 −1 1 24.2 ± 1.3
8 1 1 −1 −1 −1 1 −1 1 1 −1 89.9 ± 3.8
9 1 1 1 −1 −1 −1 1 −1 1 1 38.4 ± 1.5
10 −1 1 1 1 −1 −1 −1 1 −1 1 23.8 ± 1.1
11 1 −1 1 1 1 −1 −1 −1 1 −1 19.5 ± 0.8
12 −1 −1 −1 −1 −1 −1 −1 −1 −1 −1 56.2 ± 3.3
13 0 0 0 0 0 0 0 0 0 0 45.4 ± 1.7
14 0 0 0 0 0 0 0 0 0 0 41.8 ± 1.9
15 0 0 0 0 0 0 0 0 0 0 39.9 ± 1.6
16 −1 −1 1 −1 −1 −1 1 1 1 −1 41.5 ± 2.3
17 1 −1 −1 1 −1 −1 −1 1 1 1 20.1 ± 0.7
18 −1 1 −1 −1 1 −1 −1 −1 1 1 56.4 ± 3.0
19 1 −1 1 −1 −1 1 −1 −1 −1 1 21.8 ± 1.1
20 1 1 −1 1 −1 −1 1 −1 −1 −1 58.4 ± 2.9
21 1 1 1 −1 1 −1 −1 1 −1 −1 71.4 ± 3.7
22 −1 1 1 1 −1 1 −1 −1 1 −1 71.8 ± 4.3
23 −1 −1 1 1 1 −1 1 −1 −1 1 8.8 ± 0.2
24 −1 −1 −1 1 1 1 −1 1 −1 −1 45.3 ± 1.9
25 1 −1 −1 −1 1 1 1 −1 1 −1 42.0 ± 2.2
26 −1 1 −1 −1 −1 1 1 1 −1 1 52.1 ± 3.5
27 1 1 1 1 1 1 1 1 1 1 47.5 ± 2.4
28 0 0 0 0 0 0 0 0 0 0 47.0 ± 3.2
29 0 0 0 0 0 0 0 0 0 0 41.6 ± 1.8
30 0 0 0 0 0 0 0 0 0 0 40.5 ± 1.5
  1. Note: I, Sucrose; J, NaNO3; K, K2HPO4; L, KH2PO4; M, KCl; N, MgSO4.7H2O; O, CaCl2.2H2O; P, Na3C6H5O7.2H2O; Q, Biotin; R, Trace elements.
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