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Table 2 Experimental design using the Plackett-Burman method with AMVL production for the complex medium

From: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae

Run Coded values AMVL (mg/L)
A B C D E F G H
1 1 1 −1 1 1 1 −1 −1 161.1 ± 7.2
2 −1 1 1 −1 1 1 1 −1 139.1 ± 5.3
3 1 −1 1 1 −1 1 1 1 17.6 ± 0.3
4 −1 1 −1 1 1 −1 1 1 68.3 ± 4.7
5 −1 −1 1 −1 1 1 −1 1 50.7 ± 3.1
6 −1 −1 −1 1 −1 1 1 −1 1.4 ± 0.1
7 1 −1 −1 −1 1 −1 1 1 12.4 ± 0.5
8 1 1 −1 −1 −1 1 −1 1 81.0 ± 3.6
9 1 1 1 −1 −1 −1 1 −1 123.8 ± 5.8
10 −1 1 1 1 −1 −1 −1 1 55.0 ± 3.2
11 1 −1 1 1 1 −1 −1 −1 39.2 ± 1.6
12 −1 −1 −1 −1 −1 −1 −1 −1 1.1 ± 0.1
13 0 0 0 0 0 0 0 0 80.5 ± 4.4
14 0 0 0 0 0 0 0 0 86.2 ± 4.7
15 0 0 0 0 0 0 0 0 81.2 ± 3.5
16 −1 −1 1 −1 −1 −1 1 1 34.4 ± 2.0
17 1 −1 −1 1 −1 −1 −1 1 1.6 ± 0.2
18 −1 1 −1 −1 1 −1 −1 −1 74.8 ± 3.4
19 1 −1 1 −1 −1 1 −1 −1 48.1 ± 2.2
20 1 1 −1 1 −1 −1 1 −1 86.0 ± 4.1
21 1 1 1 −1 1 −1 −1 1 136.1 ± 5.7
22 −1 1 1 1 −1 1 −1 −1 80.9 ± 3.8
23 −1 −1 1 1 1 −1 1 −1 57.2 ± 2.3
24 −1 −1 −1 1 1 1 −1 1 2.4 ± 0.3
25 1 −1 −1 −1 1 1 1 −1 25.0 ± 0.9
26 −1 1 −1 −1 −1 1 1 1 44.5 ± 2.3
27 1 1 1 1 1 1 1 1 142.9 ± 6.8
28 0 0 0 0 0 0 0 0 92.8 ± 3.7
29 0 0 0 0 0 0 0 0 76.9 ± 2.9
30 0 0 0 0 0 0 0 0 87.1 ± 3.3
  1. Note: A, Sucrose; B, NaNO3; C, Yeast extract; D, KH2PO4; E, K2HPO4; F, KCl; G, MgSO4.7H2O; H, Trace elements.