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Table 2 Experimental design using the Plackett-Burman method with AMVL production for the complex medium

From: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae

Run

Coded values

AMVL (mg/L)

A

B

C

D

E

F

G

H

1

1

1

−1

1

1

1

−1

−1

161.1 ± 7.2

2

−1

1

1

−1

1

1

1

−1

139.1 ± 5.3

3

1

−1

1

1

−1

1

1

1

17.6 ± 0.3

4

−1

1

−1

1

1

−1

1

1

68.3 ± 4.7

5

−1

−1

1

−1

1

1

−1

1

50.7 ± 3.1

6

−1

−1

−1

1

−1

1

1

−1

1.4 ± 0.1

7

1

−1

−1

−1

1

−1

1

1

12.4 ± 0.5

8

1

1

−1

−1

−1

1

−1

1

81.0 ± 3.6

9

1

1

1

−1

−1

−1

1

−1

123.8 ± 5.8

10

−1

1

1

1

−1

−1

−1

1

55.0 ± 3.2

11

1

−1

1

1

1

−1

−1

−1

39.2 ± 1.6

12

−1

−1

−1

−1

−1

−1

−1

−1

1.1 ± 0.1

13

0

0

0

0

0

0

0

0

80.5 ± 4.4

14

0

0

0

0

0

0

0

0

86.2 ± 4.7

15

0

0

0

0

0

0

0

0

81.2 ± 3.5

16

−1

−1

1

−1

−1

−1

1

1

34.4 ± 2.0

17

1

−1

−1

1

−1

−1

−1

1

1.6 ± 0.2

18

−1

1

−1

−1

1

−1

−1

−1

74.8 ± 3.4

19

1

−1

1

−1

−1

1

−1

−1

48.1 ± 2.2

20

1

1

−1

1

−1

−1

1

−1

86.0 ± 4.1

21

1

1

1

−1

1

−1

−1

1

136.1 ± 5.7

22

−1

1

1

1

−1

1

−1

−1

80.9 ± 3.8

23

−1

−1

1

1

1

−1

1

−1

57.2 ± 2.3

24

−1

−1

−1

1

1

1

−1

1

2.4 ± 0.3

25

1

−1

−1

−1

1

1

1

−1

25.0 ± 0.9

26

−1

1

−1

−1

−1

1

1

1

44.5 ± 2.3

27

1

1

1

1

1

1

1

1

142.9 ± 6.8

28

0

0

0

0

0

0

0

0

92.8 ± 3.7

29

0

0

0

0

0

0

0

0

76.9 ± 2.9

30

0

0

0

0

0

0

0

0

87.1 ± 3.3

  1. Note: A, Sucrose; B, NaNO3; C, Yeast extract; D, KH2PO4; E, K2HPO4; F, KCl; G, MgSO4.7H2O; H, Trace elements.