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Table 1 The levels of independent variables in the Plackett-Burman Design for the complex and defined media

From: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae

Varible Components Levels of variable
−1 (Low) 0 (Central) +1 (High)
  Complex medium    
A Sucrose (g/L) 20 40 60
B NaNO3 (g/L) 1 3 5
C Yeast extract (g/L) 0 2 4
D KH2PO4 (g/L) 0 1 2
E K2HPO4 (g/L) 1 3 5
F KCl (g/L) 0.5 0.75 1
G MgSO4.7H2O (g/L) 0.5 0.75 1
H Trace elements (mL/L) 0.5 0.75 1
  Defined medium    
I Sucrose (g/L) 40 70 100
J NaNO3 (g/L) 3 5 7
K K2HPO4 (g/L) 3 5 7
L KH2PO4 (g/L) 0 1 2
M KCl (g/L) 0.25 0.50 0.75
N MgSO4.7H2O (g/L) 0.25 0.50 0.75
O CaCl2.2H2O (g/L) 0 0.10 0.20
P Na3C6H5O7.2H2O (g/L) 0 1 2
Q Biotin (mL/L) 0 0.10 0.20
R Trace elements (mL/L) 0.25 0.50 0.75