Skip to main content
Figure 2 | AMB Express

Figure 2

From: Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation

Figure 2

Following the fate of the T-DNA in pABr1-transformed hyphae. a) Sections (3 x 3 mm) of transformed T. borchii mycelia analyzed by confocal microscopy (GFP wavelength) at the indicated times after Agrobacterium infection. b) Histogram representation of cumulative data produced by the experiments in panel a) expressed as percentage of fluorescent hyphae with respect to the total number of hyphae present in each section (~4500). Data are the mean ± s.e.m. of at least five independent experiments. c) PCR amplification with sgfp specific-primers of genomic DNA obtained from mock-transformed mycelia (WT) using 0.1 μg (lane 3) and 1 μg (lane 4) of template DNA, and from AGL-1/pABr1-transformed mycelia (0.1 μg of template DNA; lane5). A PCR negative control (no DNA) and size markers (λ/HIII) are shown in the two leftmost lanes. d) Schematic representation of the probes (P1 and P2) utilized for DNA blot analysis. e) DNA blot analysis of DNA extracted from AGL-1/pABr1-transformed mycelia (“transformant”, lane 1), mock-transformed mycelia (WT, lane 2), and AGL-1/ABr-1 bacterial cells (pABr1, lane 3), hybridized with the P1 probe, which detects T-DNA integration regions. After stripping, the same membrane was hybridized with the P2 control probe.

Back to article page