Biofilm dispersal by molsidomine. Exposure to molsidomine doses ranging from 10 μM to 10pM induced biofilm dispersal as measured by staining of the attached cells with crystal violet. “0” indicates control, an existing biofilm not treated with molsidomine. A. Biofilm formed by S. enterica sv. Typhimurium 14028 in wells of a microtiter plate before treatment with molsidomine. Attached cells are visualized with crystal violet staining. B. Residual biofilm after treatment with 10 nM of molsidomine. C→H. Induction of biofilm dispersal by molsidomine. Biofilms were pre-formed on polystyrene. Contact time and temperature at which biofilms were exposed to the chemical are listed above each panel. Concentrations of molsidomine are indicated on the x-axis. Error bars are standard errors.