Effect of increasing amounts of CBM-SUMO or CBM
on filter paper hydrolysis. The ratios of CBM-SUMO to enzyme varied from 70:1 to 1:100 (w/w). Results from CBMCBHI and CBM-SUMO are grouped based on the molar concentration of those molecules in the reaction. The results are expressed as percentages of the reaction yields in presence of CBM-SUMO or CBMCBHI relative to a negative control experiment carried out without addition of one of these auxiliary proteins. The best enhancement observed was 30%. The cellulase activity after 1 h reaction was around 1 FPU/g of substrate and the enzyme concentration used was A) 33.3 μg/mL of TrCBHI, B) 58,7 μg/mL of CDCBHI and C) 27,5 μg/mL of Accellerase® 1500.