Figure 1
From: Family 1 carbohydrate binding-modules enhance saccharification rates
Expression and purification of CBM-SUMO expressed in E.coli Rosetta (DE3). Lane 1, molecular weight standard; lane 2, lysate soluble fraction from E. coli induced with IPTG; lane 3, proteins that did not bind to Ni-NTA resin; lane 4, proteins eluted from Ni-NTA resin with wash buffer; lane 5, protein eluted from Ni-NTA resin with 300 mM imidazole; lane 6, pure CBM-SUMO eluted from size-exclusion chromatography.