Table 1 Oligonucleotides used during the study
From: Erythritol production on wheat straw using Trichoderma reesei
Name | Sequence (5’ – 3’) | Usage |
|---|---|---|
pbxl1_SalI__EcoRI_f | ATATAGTCGACGAATTCAGCTTGTCTGCCTTGATTACCATCC | Vector construction |
pbxl1_XbaI_r | ATATATCTAGATGCGTCCGGCTGTCCTTC | Vector construction |
err1_XbaI_f | ATATATCTAGAATGTCTTCCGGAAGGACC | Vector construction |
err1_Nsi_r | TATATATGCATTTACAGCTTGATGACAGCAGTG | Vector construction |
ppki_f | GCACGCATCGCCTTATCGTC | PCR test |
qerr1_f | CTTTACCATTGAGCACCTCGACG | RT-qPCR |
qerr1_r | GGTCTTGCCCTGCTTCTTGG | RT-qPCR |
qact1_f | TGAGAGCGGTGGTATCCACG | RT-qPCR |
qact1_r | GGTACCACCAGACATGACAATGTTG | RT-qPCR |
qsar1_f | TGGATCGTCAACTGGTTCTACGA | RT-qPCR |
qsar1_r | GCATGTGTAGCAACGTGGTCTTT | RT-qPCR |