Figure 2

Southern blot analysis of parental and err1 overexpression T. reesei strains. On an agarose gel Nde I/Sal I/Bgl II-digested DNA from the wild-type (WT) strain bearing the native err1 (2340 bp), the thereof derived err1 overexpression strains QPEC1 (containing the native err1 (2340 bp) and n+1 inserted fragments (5410 bp)) and QBEC2 (containing the native err1 (2340 bp) and n+1 inserted fragments (6626 bp)), Rut-C30 bearing the native err1 (2340 bp), and the thereof derived err1 overexpression strains RPEC1 (containing the native err1 (2340 bp) and n+1 inserted fragments (5410 bp)) and RBEC2 (containing the native err1 (2340 bp) and n+1 inserted fragments (6626 bp)) was separated. n means the band intensity in relation to the native err1-containing band. A 1 kb DNA ladder (L) was used for estimation of DNA fragment size; indicated sizes are given in bp. As probe a biotin-labeled fragment containing the structural err1 gene was used. For visualization Dylight 650-labeled streptavidin was applied and the membrane was scanned with a Typhoon FLA 9500.