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AMB Express

Table 1 Results of the spectrophotometric assay

From: Michael hydratase alcohol dehydrogenase or just alcohol dehydrogenase?

Entry

Experiment

Δ Abs/min[a]

1

DCPIP + buffer

0.0003

2

DCPIP + 3

-0.0008

3

DCPIP + 4

-0.0012

4

DCPIP + TADH

-0.0001

5

DCPIP + MhyADH

-0.0007

6

DCPIP + 3 + MhyADH

-0.0087

7

DCPIP + 4 + MhyADH

-0.0080

8

DCPIP + 3 + TADH

-0.0199

  1. [a] Decrease of absorbance is given as Δ absorbance unit per min. 3: 2-cyclohexenone, 4: 3-hydroxycyclohexanone. The graph to the data shown in the table can be found in the Additional file 1.
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