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Figure 3 | AMB Express

Figure 3

From: Michael hydratase alcohol dehydrogenase or just alcohol dehydrogenase?

Figure 3

Principle of the spectrophotometric assay. The water addition to 3 is taking place spontaneously and the formed 4 is further converted by the ADH activity. The oxidised state of DCPIP is blue, the reduced colourless. DCPIP serves as an electron acceptor for the ADH, and its reduction can be detected as a decrease of absorbance. Results from the spectrophotometric assay using DCPIP are given in Table 1.

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