Table 1 Summary of nucleotide primers used in this study
From: Xylanase (GH11) from Acremonium cellulolyticus: homologous expression and characterization
Primer | Nucleotide sequence (5’ → 3’) |
|---|---|
For plasmids construction | |
AC-F12 (xylA) | ATTGTTAACAAGATGAAGATCACATCAGTGTTCG |
AC-R12 (xylA) | AATCCTGCAGGTTAAGATACAGTAACAGTGGCACTTC |
AC-F13 (xylB) | ATTGTTAACATCATGGGCATCTCATCTATTCTTC |
AC-R13 (xylB) | AATCCTGCAGGCTATTGGCACTGACTGTAGTAAGCGT |
AC-F25 (xylC) | ATTGTTAACAAGATGAAGCTCTCTCTGGCTGCAA |
AC-R25 (xylC) | AATCCTGCAGGCTAGGACACGGTGATGGTACTAGAAC |
AC-F26 (xylD) | ATTGTTAACAAGATGCGGTCATTTGCTCGCCTTGTC |
AC-R26 (xylD) | AATCCTGCAGGTCAGCTAACAGTAAAATCCAGGTAAC |
AC-F27 (xylE) | ATTGTTAACAAGATGATTTATTTCCCTCAGCTCATG |
AC-R27 (xylE) | AATCCTGCAGGCTATTGAGTGGCAGTCTGCTGGGCA |
AC-F28 (xylF) | ATTGTTAACAAGATGTTCTCTTTCAGTACTGCCTT |
AC-R28 (xylF) | AATCCTGCAGGCTACAAGCATTGATAGTAGTACGGGT |
AC-F29 (xylG) | ATTGATATCAAGATGGTTGCTTTCTCGAGCTTATTTAC |
AC-R29 (xylG) | AATCCTGCAGGGTCCAACATCAATGCTACTTACAGC |
For quantitative PCR | |
xylA-f | GAGGACGGAGTCAATGGAGA |
xylA-r | CCGAGAGGTAGGAGCCAGAA |
xylB-f | TGCTCTCGGTGTTGATGTTG |
xylB-r | GTGGTCTGGTAGTCGGTGGA |
xylC-f | GTGTCGACCAACCCTCCATC |
xylC-r | GTCCAAGTGCCTTCCATGCT |
xylD-f | TTTAGCGATAGCGGCAGGTT |
xylD-r | CCCATGTAAGGGAGCGAGTT |
xylE-f | CGGGCCACAAACTATATCCA |
xylE-r | TTCCAGCCAACACCAACAAC |
xylF-f | ACTAGCAAGGACGGCGTAGA |
xylF-r | CACCGAGGAACTCAGACGAA |
xylG-f | CAGCACGGGGTTTGAGGTT |
xylG-r | CCGAAGTTGATGGGAGTGGT |
gpdA-f | AACATCATTCCCAGCAGCAC |
gpdA-r | CGGCAGGTCAAGTCAACAAC |