Cloning, expression and characterization of a versatile Baeyer-Villiger monooxygenase from Dietzia sp. D5

AMB Express

Table 2 Relative activity of BVMO4 towards different substrates

Substrates	Relative activity %
Alicyclic ketones
Cyclobutanone	20.2 ± 6.1
Cyclopentanone	12.7 ± 5.9
Cyclohexanone	22.0 ± 3.5
Cycloheptanone	12.0 ± 3.6
Cyclooctanone	9.3 ± 3.9
Cyclopentadecanone	0.0
Substituted alicyclic ketones
2-methylcyclohexanone	155.3 ± 24.8
3-methylcyclohexanone	90.5 ± 1.9
4-methylcyclohexanone	14.6 ± 0.7
2-cyclohexen-1-one	5.4 ± 1.1
Bicyclic ketones
Bicyclo[3.2.0]hept-2-en-6-one	103.0 ± 6.4
Norcamphor	6.3 ± 2.2
Beta tetralone	0.0
Alpha tetralone	10.8 ± 2.7
Aliphatic ketones
2-heptanone	20.7 ± 0.7
2-octanone	22.6 ± 1.7
2-decanone	22.0 ± 0.4
2-pentadecanone	20.9 ± 3.5
Aliphatic ketone with aromatic substituents
Phenylacetone	100.0 ± 6.2
Acetophenone	2.4 ± 0.8
4′-hydroxyacetophenone	0.0
Benzaldehyde	0.0
Sulfides
Thioanisole	33.0 ± 13.5
Ethionamide	32.0 ± 1.8
Other ketones
Progesterone^a	23.0 ± 0.6
Estrone^a	17.0 ± 0.4
Testosterone^a	6.6 ± 2.4
Menthone	77.1 ± 2.7

The activity on phenylacetone which is considered as 100% was 1.87 mU. The initial substrate concentration was 5 mM. ^aSubstrate concentration was 0.5 mM.