Figure 3
Effect of pH on activity and stability of BVMO4. a) Effect of pH on the activity of BVMO4 was determined by assaying the enzyme at 25°C in 50 mM sodium acetate buffer (▲) pH 5 to 5.5, sodium phosphate buffer (■) pH 6 to 8, and Tris–HCl (♦) pH 7.5 to 9. b) The residual activity of BVMO4 after 24 days at 4 °C and different pH values was measured at 25°C in 50 mM sodium acetate (▲), sodium phosphate (■) and Tris–HCl (♦) buffers.