Disruption of pknG enhances production of gamma-aminobutyric acid by Corynebacterium glutamicum expressing glutamate decarboxylase

AMB Express

Table 2 Oligonucleotide primers

Primer name	Sequence (5′- 3′)	Restriction enzyme
pknG-up-In-F	GCCAAGCTTgcatgcATGAAGGATAATGAAGATTTCGATCCAGATTCACCAGC	Sph I
pknG-up-In-R-801	ACCATTTGTGTCGCC GGCTTTGCAGCGGTCTTTCAGGGA
pknG-down-In-F-1669	GCCAAGCTTgcatgcGGCGACACAAATGGT TCTCCG	Sph I
pknG-down-In-R	AAAAGGATCggatccCTAGAACCAACTCAGTGGCCGCA	Bam HI
pknG-In-F2	CCAGTGCCAAGCTTgcatgcATGAAGGATAATGAAGATTTCGATCCAGATTCACCAGC	Sph I
pknG-In-R2	AAAAAGGATCggatccCTAGAACCAACTCAGTGGCCGCA	Bam HI
SacI-gadB-F	GGCgagctcATGTTTAAAGCTGTTCTGTTGGGCAA	Sac I
XhoI-gadBF-R	CCGctcgagTTACTTGTCATCGTCATCCTTGTAGTCAGGTCGGAACTACTCGATTCACG	Xho I

Restriction enzyme cleavage sites are shown in small letters, and complementary sequences of the primer pairs used for overlap-extension PCR are shown in italics.