Efficient fermentation of an improved synthetic grape must by enological and laboratory strains of Saccharomyces cerevisiae

AMB Express

Table 1 Macrokinetic parameters for fermentations of NGM, RB-SGM and ISA-SGM by ISA1000, S288C and BY4741 strains

Medium	Supp. additionª	Strain	Lag-phase (h)	Doubling time (h)	Final OD	Minimum [Glucose] (g/L)	AF1^b(h)	AF2^b(h)	Complete fermentation
NGM	--	ISA1000	<2	2.4 ± 0.18	21.2 ± 0.76	0	142	166	YES
	--	S288C	<2	3.3 ± 0.12	20.4 ± 1.87	0	70	150	YES
	--	BY4741	4 ± 0.67	7.8 ± 0.11	8.8 ± 0.43	0	476	530	YES
	1x	BY4741	<2	3.9 ± 0.18	15.3 ± 0.83	0	476	528	YES
	2x	BY4741	<2	3.3 ± 0.20	19.6 ± 1.42	0	145	152	YES
RB-SGM	--	S288C	40 ± 0.84	3.4 ± 0.21	10.4 ± 0.88	35	>570	>570	NO
ISA-SGM	--	ISA1000	3 ± 0.75	2.6 ± 0.15	23.4 ± 0.64	0	106	150	YES
	2x	ISA1000	4 ± 0.74	1.9 ± 0.12	22.6 ± 0.74	0	105	140	YES
	--	S288C	9 ± 1.71	3.4 ± 0.21	24.2 ± 1.61	0	159	183	YES
	2x	S288C	8 ± 0.71	2.3 ± 0.11	23.3 ± 0.97	0	150	165	YES
	2x	BY4741	9 ± 0.62	3.6 ± 0.26	23.6 ± 0.83	0	159	300	YES

Data represent the average of data from at least two independent cultures.
Standard deviation calculated from duplicate experiments (± standard deviation).
^aConcentration of auxotrophic supplements approximately corresponding to those described by Pronk ([2002]).
^bAF1 (Alcoholic Fermentation for Glucose) and AF2 (Alcoholic Fermentation for Fructose) are the time for glucose and fructose fermentation excluding the lag-phase, respectively.