AMB Express

Table 1 mRNA transcription levels of pca genes

From: Artificial oxidative stress-tolerant Corynebacterium glutamicum

Target gene	RNA-seq^a(RPKM)	RT-qPCR^b(relative fold)
Target gene	WT	WT	+*pcaIJ*	+*pcaFD*	+*pcaHGBC*
pcaI	135.7	1.00 ± 0.39	1.75 ± 0.36	1.55 ± 0.50	0.61 ± 0.17
pcaJ	134.8	1.00 ± 0.15	1.23 ± 0.14	2.56 ± 0.27	1.10 ± 0.12
pcaF	220.9	1.00 ± 0.05	0.24 ± 0.03	40.2 ± 6.6	1.00 ± 0.15
pcaD	183.6	1.00 ± 0.18	0.25 ± 0.05	42.5 ± 5.9	1.00 ± 0.13
pcaH	172.0	1.00 ± 0.02	0.23 ± 0.01	2.61 ± 0.12	10.1 ± 0.95
pcaG	116.7	1.00 ± 0.01	0.23 ± 0.02	2.45 ± 0.16	8.83 ± 0.60
pcaB	217.5	1.00 ± 0.22	0.19 ± 0.04	2.31 ± 0.39	7.72 ± 0.42
pcaC	77.7	1.00 ± 0.02	0.21 ± 0.02	2.52 ± 0.23	11.9 ± 0.71

The cells were grown in MCGC minimal medium without H₂O₂-stress and harvested in the log phase for mRNA preparation.
^aAdapted from (Lee et al. [2013b]) (NCBI Gene Expression Omnibus access code: GSE41232) RPKM (reads per kilo base per million).
^bThis study. Values are mean ± SD from three independent experiments.

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