From: Structural basis of stereospecific reduction by quinuclidinone reductase
Mutation | Relative activity for 3-qinuclidinonea | Km(mM)b | Vmax(unit/mg)a | kcat(sec-1) | |
---|---|---|---|---|---|
WT | 100 | 440 | 31.2 | 15.1 | |
Catalytic site | S166A | ND | |||
Y181A | ND | ||||
Y181F | ND | ||||
K185A | ND | ||||
Substrate-binding site | I167A | ND | |||
I167V | 24.5 | 308 | 5.56 | 2.69 | |
S168A | 115.7 | 168 | 19.9 | 9.64 | |
N173A | 97.8 | 100 | 11.6 | 5.62 | |
Q178A | 164.5 | 103 | 20.2 | 9.79 | |
F212A | ND | ||||
F212L | ND |